EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.21.107 | expressed in Escherichia coli as a His-tagged fusion protein | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.21.107 | C57A/C69A | mutant represents a completely reduced HtrA being unable to form the intramolecular S-S bond. Mutant very efficiently degrades alkaline phosphatase at 20°C which is very pronounced compared to wild-type. Thus, the reduction of HtrAs disulfide bridge may facilitate the activation of the protease | Escherichia coli |
3.4.21.107 | additional information | a htrAdsbA double-mutant, lacking the functional htrA gene and the functional DsbA/DsbB oxidoreductase system shows a dramatically inhibited growth rate in the presence of 7 mM DTT when compared to mutant strain dsbA containing a single mutation in the DsbA/DsbB oxidoreductase system, indicating that htrA gene is important for survival of the dsbA mutant in the reducing environment | Escherichia coli |
3.4.21.107 | S210A | mutant S210A, lacking proteolytic activity but retaining chaperone activity is overexpressed in mutant strain htrAdsbA, lacking the functional htrA gene and the functional DsbA/DsbB oxidoreductase system, which: the presence of mutant S210A increases the survival rate of the htrAdsbA double-mutant bacteria. Thus, the proteolytic activity of HtrA seems to play an important role in bacterial cells even at low temperatures (30°C), at which the chaperone function is proposed as being dominant | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.107 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.107 | using Ni-NTA chromatography | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.107 | reduced alkaline phosphatase + H2O | - |
Escherichia coli | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.107 | DegP | - |
Escherichia coli |
3.4.21.107 | HtrA | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.107 | 20 | - |
reduced alkaline phosphatase is efficiently degraded at 20°C, both in vivo and in vitro. The cleavage is most efficient in the case of a C57A/C69A mutant, lacking its internal SS bond | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.107 | 8 | - |
assay at | Escherichia coli |