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Literature summary extracted from

  • Skorko-Glonek, J.; Sobiecka-Szkatula, A.; Narkiewicz, J.; Lipinska, B.
    The proteolytic activity of the HtrA (DegP) protein from Escherichia coli at low temperatures (2008), Microbiology, 154, 3649-3658.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.107 expressed in Escherichia coli as a His-tagged fusion protein Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.107 C57A/C69A mutant represents a completely reduced HtrA being unable to form the intramolecular S-S bond. Mutant very efficiently degrades alkaline phosphatase at 20°C which is very pronounced compared to wild-type. Thus, the reduction of HtrA’s disulfide bridge may facilitate the activation of the protease Escherichia coli
3.4.21.107 additional information a htrAdsbA double-mutant, lacking the functional htrA gene and the functional DsbA/DsbB oxidoreductase system shows a dramatically inhibited growth rate in the presence of 7 mM DTT when compared to mutant strain dsbA containing a single mutation in the DsbA/DsbB oxidoreductase system, indicating that htrA gene is important for survival of the dsbA mutant in the reducing environment Escherichia coli
3.4.21.107 S210A mutant S210A, lacking proteolytic activity but retaining chaperone activity is overexpressed in mutant strain htrAdsbA, lacking the functional htrA gene and the functional DsbA/DsbB oxidoreductase system, which: the presence of mutant S210A increases the survival rate of the htrAdsbA double-mutant bacteria. Thus, the proteolytic activity of HtrA seems to play an important role in bacterial cells even at low temperatures (30°C), at which the chaperone function is proposed as being dominant Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.107 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.107 using Ni-NTA chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.107 reduced alkaline phosphatase + H2O
-
Escherichia coli ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.107 DegP
-
Escherichia coli
3.4.21.107 HtrA
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.107 20
-
reduced alkaline phosphatase is efficiently degraded at 20°C, both in vivo and in vitro. The cleavage is most efficient in the case of a C57A/C69A mutant, lacking its internal S–S bond Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.107 8
-
assay at Escherichia coli