EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.55 | overexpression in Escherichia coli BL21(DE3) | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.55 | 0.36 | - |
linear alpha-1,5-L-arabinan | - |
Bacillus subtilis | |
3.2.1.55 | 0.42 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Bacillus subtilis | |
3.2.1.55 | 0.49 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Bacillus subtilis | |
3.2.1.55 | 0.78 | - |
1,5-alpha-L-arabinobiose | - |
Bacillus subtilis | |
3.2.1.55 | 1.1 | - |
1,5-alpha-L-arabinotriose | - |
Bacillus subtilis | |
3.2.1.55 | 2.5 | - |
alpha-1,5-L-arabinotriose | - |
Bacillus subtilis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.55 | cytoplasm | - |
Bacillus subtilis | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.55 | additional information | the addition of the chelating agent EDTA does not affect the activity of AbfA, suggesting that no metals are needed for the enzymic reaction | Bacillus subtilis | |
3.2.1.55 | additional information | the addition of the chelating agent EDTA does not affect the activity of either Abf2, suggesting that no metals are needed for the enzymic reaction | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 58000 | - |
2 * 58000, SDS-PAGE | Bacillus subtilis |
3.2.1.55 | 112000 | - |
Abf2 is present in two oligomeric states, a predominant form with an estimated molecular mass of 112 kDa that could correspond to the dimer and an additional oligomeric form of about 276 kDa | Bacillus subtilis |
3.2.1.55 | 116000 | - |
gradient native PAGE, two oligomeric forms are observed, the dimer (116 kDa) and an oligomer with a higher-order structure than the tetramer | Bacillus subtilis |
3.2.1.55 | 276000 | - |
Abf2 is present in two oligomeric states, a predominant form with an estimated molecular mass of 112 kDa that could correspond to the dimer and an additional oligomeric form of about 276 kDa | Bacillus subtilis |
3.2.1.55 | 296000 | - |
gradient native PAGE, two oligomeric forms are observed, the dimer (116 kDa) and an oligomer with a higher-order structure than the tetramer | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.55 | Bacillus subtilis | P94531 | - |
- |
3.2.1.55 | Bacillus subtilis | P94552 | - |
- |
3.2.1.55 | Bacillus subtilis 168T+ | P94531 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.55 | Ni-NTA agarose affinity chromatography | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.55 | 1,5-alpha-L-arabinobiose + H2O | - |
Bacillus subtilis | ? | - |
? | |
3.2.1.55 | 1,5-alpha-L-arabinobiose + H2O | - |
Bacillus subtilis 168T+ | ? | - |
? | |
3.2.1.55 | 1,5-alpha-L-arabinotriose + H2O | - |
Bacillus subtilis | ? | - |
? | |
3.2.1.55 | 1,5-alpha-L-arabinotriose + H2O | - |
Bacillus subtilis 168T+ | ? | - |
? | |
3.2.1.55 | alpha-1,5-L-arabinotriose + H2O | - |
Bacillus subtilis | ? | - |
? | |
3.2.1.55 | linear alpha-1,5-L-arabinan + H2O | - |
Bacillus subtilis | ? | - |
? | |
3.2.1.55 | linear alpha-1,5-L-arabinan + H2O | - |
Bacillus subtilis 168T+ | ? | - |
? | |
3.2.1.55 | additional information | Abf2 is most active on (1-2) and (1-3) linkages of branched arabinan and arabinoxylan | Bacillus subtilis | ? | - |
? | |
3.2.1.55 | additional information | AbfA acts preferentially on (1-5) linkages of linear alpha-1,5-L-arabinan and alpha-1,5-linked arabino-oligomers, and is much less effective on branched sugar beet arabinan and arabinoxylan and arabinogalactan | Bacillus subtilis | ? | - |
? | |
3.2.1.55 | additional information | AbfA acts preferentially on (1-5) linkages of linear alpha-1,5-L-arabinan and alpha-1,5-linked arabino-oligomers, and is much less effective on branched sugar beet arabinan and arabinoxylan and arabinogalactan | Bacillus subtilis 168T+ | ? | - |
? | |
3.2.1.55 | p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | p-nitrophenol + L-arabinofuranose | - |
? | |
3.2.1.55 | p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis 168T+ | p-nitrophenol + L-arabinofuranose | - |
? | |
3.2.1.55 | sugar beet arabinan (branched) + H2O | - |
Bacillus subtilis | ? | - |
? | |
3.2.1.55 | wheat arabinoxylan + H2O | - |
Bacillus subtilis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.55 | dimer | 2 * 58000, SDS-PAGE | Bacillus subtilis |
3.2.1.55 | oligomer | higher than the tetramer, subunit mass 58000 | Bacillus subtilis |
3.2.1.55 | oligomer | two oligomeric forms are observed, the dimer (116 kDa) and an oligomer with a higher-order structure than the tetramer, subunit mass 58000 | Bacillus subtilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.55 | Abf2 | - |
Bacillus subtilis |
3.2.1.55 | AbfA | - |
Bacillus subtilis |
3.2.1.55 | AFS | - |
Bacillus subtilis |
3.2.1.55 | alpha-L-arabinofuranosidase | - |
Bacillus subtilis |
3.2.1.55 | alpha-L-arabinofuranoside arabinofuranohydrolase | - |
Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 50 | - |
- |
Bacillus subtilis |
3.2.1.55 | 60 | - |
- |
Bacillus subtilis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 30 | 80 | - |
Bacillus subtilis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 55 | - |
AbfA has a half-life of about 17.6 min at 55°C | Bacillus subtilis |
3.2.1.55 | 70 | - |
Abf2 has a half-life of about 9.34 min at 70°C | Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.55 | 0.75 | - |
1,5-alpha-L-arabinotriose | - |
Bacillus subtilis | |
3.2.1.55 | 12 | - |
linear alpha-1,5-L-arabinan | - |
Bacillus subtilis | |
3.2.1.55 | 32 | - |
1,5-alpha-L-arabinobiose | - |
Bacillus subtilis | |
3.2.1.55 | 88 | - |
1,5-alpha-L-arabinotriose | - |
Bacillus subtilis | |
3.2.1.55 | 295 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Bacillus subtilis | |
3.2.1.55 | 306 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 8 | - |
- |
Bacillus subtilis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 3 | 9 | - |
Bacillus subtilis |