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Literature summary extracted from

  • Inacio, J.M.; Correia, I.L.; de Sa-Nogueira, I.
    Two distinct arabinofuranosidases contribute to arabino-oligosaccharide degradation in Bacillus subtilis (2008), Microbiology, 154, 2719-2729.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 overexpression in Escherichia coli BL21(DE3) Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.36
-
linear alpha-1,5-L-arabinan
-
Bacillus subtilis
3.2.1.55 0.42
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Bacillus subtilis
3.2.1.55 0.49
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Bacillus subtilis
3.2.1.55 0.78
-
1,5-alpha-L-arabinobiose
-
Bacillus subtilis
3.2.1.55 1.1
-
1,5-alpha-L-arabinotriose
-
Bacillus subtilis
3.2.1.55 2.5
-
alpha-1,5-L-arabinotriose
-
Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.55 cytoplasm
-
Bacillus subtilis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.55 additional information the addition of the chelating agent EDTA does not affect the activity of AbfA, suggesting that no metals are needed for the enzymic reaction Bacillus subtilis
3.2.1.55 additional information the addition of the chelating agent EDTA does not affect the activity of either Abf2, suggesting that no metals are needed for the enzymic reaction Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 58000
-
2 * 58000, SDS-PAGE Bacillus subtilis
3.2.1.55 112000
-
Abf2 is present in two oligomeric states, a predominant form with an estimated molecular mass of 112 kDa that could correspond to the dimer and an additional oligomeric form of about 276 kDa Bacillus subtilis
3.2.1.55 116000
-
gradient native PAGE, two oligomeric forms are observed, the dimer (116 kDa) and an oligomer with a higher-order structure than the tetramer Bacillus subtilis
3.2.1.55 276000
-
Abf2 is present in two oligomeric states, a predominant form with an estimated molecular mass of 112 kDa that could correspond to the dimer and an additional oligomeric form of about 276 kDa Bacillus subtilis
3.2.1.55 296000
-
gradient native PAGE, two oligomeric forms are observed, the dimer (116 kDa) and an oligomer with a higher-order structure than the tetramer Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Bacillus subtilis P94531
-
-
3.2.1.55 Bacillus subtilis P94552
-
-
3.2.1.55 Bacillus subtilis 168T+ P94531
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 Ni-NTA agarose affinity chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 1,5-alpha-L-arabinobiose + H2O
-
Bacillus subtilis ?
-
?
3.2.1.55 1,5-alpha-L-arabinobiose + H2O
-
Bacillus subtilis 168T+ ?
-
?
3.2.1.55 1,5-alpha-L-arabinotriose + H2O
-
Bacillus subtilis ?
-
?
3.2.1.55 1,5-alpha-L-arabinotriose + H2O
-
Bacillus subtilis 168T+ ?
-
?
3.2.1.55 alpha-1,5-L-arabinotriose + H2O
-
Bacillus subtilis ?
-
?
3.2.1.55 linear alpha-1,5-L-arabinan + H2O
-
Bacillus subtilis ?
-
?
3.2.1.55 linear alpha-1,5-L-arabinan + H2O
-
Bacillus subtilis 168T+ ?
-
?
3.2.1.55 additional information Abf2 is most active on (1-2) and (1-3) linkages of branched arabinan and arabinoxylan Bacillus subtilis ?
-
?
3.2.1.55 additional information AbfA acts preferentially on (1-5) linkages of linear alpha-1,5-L-arabinan and alpha-1,5-linked arabino-oligomers, and is much less effective on branched sugar beet arabinan and arabinoxylan and arabinogalactan Bacillus subtilis ?
-
?
3.2.1.55 additional information AbfA acts preferentially on (1-5) linkages of linear alpha-1,5-L-arabinan and alpha-1,5-linked arabino-oligomers, and is much less effective on branched sugar beet arabinan and arabinoxylan and arabinogalactan Bacillus subtilis 168T+ ?
-
?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Bacillus subtilis p-nitrophenol + L-arabinofuranose
-
?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Bacillus subtilis 168T+ p-nitrophenol + L-arabinofuranose
-
?
3.2.1.55 sugar beet arabinan (branched) + H2O
-
Bacillus subtilis ?
-
?
3.2.1.55 wheat arabinoxylan + H2O
-
Bacillus subtilis ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 dimer 2 * 58000, SDS-PAGE Bacillus subtilis
3.2.1.55 oligomer higher than the tetramer, subunit mass 58000 Bacillus subtilis
3.2.1.55 oligomer two oligomeric forms are observed, the dimer (116 kDa) and an oligomer with a higher-order structure than the tetramer, subunit mass 58000 Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 Abf2
-
Bacillus subtilis
3.2.1.55 AbfA
-
Bacillus subtilis
3.2.1.55 AFS
-
Bacillus subtilis
3.2.1.55 alpha-L-arabinofuranosidase
-
Bacillus subtilis
3.2.1.55 alpha-L-arabinofuranoside arabinofuranohydrolase
-
Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 50
-
-
Bacillus subtilis
3.2.1.55 60
-
-
Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 30 80
-
Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 55
-
AbfA has a half-life of about 17.6 min at 55°C Bacillus subtilis
3.2.1.55 70
-
Abf2 has a half-life of about 9.34 min at 70°C Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.55 0.75
-
1,5-alpha-L-arabinotriose
-
Bacillus subtilis
3.2.1.55 12
-
linear alpha-1,5-L-arabinan
-
Bacillus subtilis
3.2.1.55 32
-
1,5-alpha-L-arabinobiose
-
Bacillus subtilis
3.2.1.55 88
-
1,5-alpha-L-arabinotriose
-
Bacillus subtilis
3.2.1.55 295
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Bacillus subtilis
3.2.1.55 306
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 8
-
-
Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 3 9
-
Bacillus subtilis