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Literature summary extracted from

  • Fasiolo,F.; Gibson, B.W.; Walter, P.; Chatton, B.; Biemann, K.; Boulanger, Y.
    Cytoplasmic methionyl-tRNA synthetase from bakers' yeast. A monomer with a post-translationally modified N-terminus (1985), J. Biol. Chem., 260, 15571-15576.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.10
-
 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.10 additional information a strain carrying the MES1 structure gene on a high copy number plasmid, pFL1 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.10 0.001
-
 tRNAMet
-
 Saccharomyces cerevisiae
6.1.1.10 0.005
-
 Met
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.10 cytoplasm
-
 Saccharomyces cerevisiae 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.10 80000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Saccharomyces cerevisiae
-
 a strain carrying the MES1 structure gene on a high copy number plasmid (pFL1)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.10
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Saccharomyces cerevisiae AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.10 monomer 1 * 80000, SDS-PAGE Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.10 3 6 Met-tRNAMet
-
 Saccharomyces cerevisiae