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Literature summary extracted from

  • Field, S.J.; Thorndycroft, F.H.; Matorin, A.D.; Richardson, D.J.; Watmough, N.J.
    The respiratory nitric oxide reductase (NorBC) from Paracoccus denitrificans (2008), Methods Enzymol., 437, 79-101.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.5 expressed in Escherichia coli JM109 cells Paracoccus denitrificans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.5 membrane NorBC is a complex integral membrane protein Paracoccus denitrificans 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.5 17000
-
 1 * 56000 + 1 * 17000 Paracoccus denitrificans
1.7.2.5 56000
-
 1 * 56000 + 1 * 17000 Paracoccus denitrificans

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.5 Paracoccus denitrificans
-
-
-
1.7.2.5 Paracoccus denitrificans 93.11
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.5 Q-Sepharose column chromatography and HiTrap chelating Sepharose column chromatography Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.5 nitric oxide + reduced N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Paracoccus denitrificans nitrous oxide + oxidized N,N,N',N'-tetramethyl-p-phenylenediamine + H2O
-
 ?
1.7.2.5 nitric oxide + reduced N,N,N',N'-tetramethyl-p-phenylenediamine
-
 Paracoccus denitrificans 93.11 nitrous oxide + oxidized N,N,N',N'-tetramethyl-p-phenylenediamine + H2O
-
 ?
1.7.2.5 nitric oxide + reduced phenazine methosulfate
-
 Paracoccus denitrificans nitrous oxide + oxidized phenazine methosulfate + H2O
-
 ?
1.7.2.5 nitric oxide + reduced phenazine methosulfate
-
 Paracoccus denitrificans 93.11 nitrous oxide + oxidized phenazine methosulfate + H2O
-
 ?
1.7.2.5 nitric oxide + reduced pseudoazurin
-
 Paracoccus denitrificans nitrous oxide + oxidized pseudoazurin + H2O
-
 ?
1.7.2.5 nitric oxide + reduced pseudoazurin
-
 Paracoccus denitrificans 93.11 nitrous oxide + oxidized pseudoazurin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.5 heterodimer 1 * 56000 + 1 * 17000 Paracoccus denitrificans

Synonyms

EC Number Synonyms Comment Organism
1.7.2.5 NorBC
-
 Paracoccus denitrificans
1.7.2.5 respiratory nitric oxide reductase
-
 Paracoccus denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.5 cytochrome NorBC is a two subunit cytochrome bc complex Paracoccus denitrificans
1.7.2.5 heme subunit NorB contains a magnetically isolated low-spin heme b Paracoccus denitrificans