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Literature summary extracted from

  • Inagaki, S.; Esaka, Y.; Deyashiki, Y.; Uno, B.; Hara, A.; Toyooka, T.
    Human liver dihydrodiol dehydrogenase 1-catalyzed reaction generating 9alpha,11beta-prostaglandin F2 from prostaglandin D2 followed by micellar electrokinetic chromatography (2008), J. Sep. Sci., 31, 735-740.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.188 recombinant enzyme is expressed in Escherichia coli Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.188 prostaglandin D2 + NADPH + H+ Homo sapiens
-
 9-alpha,11-beta-prostaglandin F2 + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.188 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.188 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.188 additional information
-
 at 37°C in 100 mM Tris/HCl (pH 7.0), the reaction product, 9-alpha,11-beta-prostaglandin F2 is detected as well as the substrate prostaglandin D2 in the reaction solution after reaction for 1 h, the amount of the product increases linearly for 1 h and then gradually plateaues Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.188 prostaglandin D2 + NADPH + H+
-
 Homo sapiens 9-alpha,11-beta-prostaglandin F2 + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.188 DD1
-
 Homo sapiens
1.1.1.188 dihydrodiol dehydrogenase 1
-
 Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.188 25 37 the activity increases gradually as the temperature increases from 25 to 37°C, and then decreases significantly at 45°C compared with that at 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.188 7
-
 in 100 mM Tris/HCl Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.188 NADPH
-
 Homo sapiens