BRENDA - Enzyme Database

X-ray crystal structure of GarR - tartronate semialdehyde reductase from Salmonella typhimurium

Osipiuk, J.; Zhou, M.; Moy, S.; Collart, F.; Joachimiak, A.; J. Struct. Funct. Genomics 10, 249-253 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.60
gene garR, expression of the His6-tagged enzyme in Escherichia coli strain BL21
Salmonella enterica subsp. enterica serovar Typhimurium
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.1.1.60
enzyme with L-tartrate bound to the active site, sitting drop vapour diffusion method, mixing of 0.0005 ml of the protein solution, containing 107 mg/ml protein in in 10 mM HEPES pH 8.0, 200 mM NaCl, with 0.0005 ml of 0.1 M imidazole buffer pH 8.0, 1 M potassium/sodium L-tartrate and 0.2 M sodium chloride, and equilibrated at 16°C over 0.135 ml of this solution, overnight, X-ray diffraction structure determination and analysis at 1.65 A resolution, the single-wavelength anomalous diffraction method
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.60
tartronate semialdehyde + NADH
Salmonella enterica subsp. enterica serovar Typhimurium
as cofactor in the final stage of D-glycerate biosynthesis
(R)-glycerate + NAD+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.60
Salmonella enterica subsp. enterica serovar Typhimurium
-
gene garR
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.60
recombinant His6-tagged GarR from Escherichia coli strain BL21, the tag is removed by TEV protease, purification by nickel affinity chromatography
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.60
tartronate semialdehyde + NADH
as cofactor in the final stage of D-glycerate biosynthesis
699856
Salmonella enterica subsp. enterica serovar Typhimurium
(R)-glycerate + NAD+
-
-
-
?
1.1.1.60
tartronate semialdehyde + NADH + H+
-
699856
Salmonella enterica subsp. enterica serovar Typhimurium
(R)-glycerate + NAD+
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.60
NADH
-
Salmonella enterica subsp. enterica serovar Typhimurium
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.60
gene garR, expression of the His6-tagged enzyme in Escherichia coli strain BL21
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.60
NADH
-
Salmonella enterica subsp. enterica serovar Typhimurium
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.1.1.60
enzyme with L-tartrate bound to the active site, sitting drop vapour diffusion method, mixing of 0.0005 ml of the protein solution, containing 107 mg/ml protein in in 10 mM HEPES pH 8.0, 200 mM NaCl, with 0.0005 ml of 0.1 M imidazole buffer pH 8.0, 1 M potassium/sodium L-tartrate and 0.2 M sodium chloride, and equilibrated at 16°C over 0.135 ml of this solution, overnight, X-ray diffraction structure determination and analysis at 1.65 A resolution, the single-wavelength anomalous diffraction method
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.60
tartronate semialdehyde + NADH
Salmonella enterica subsp. enterica serovar Typhimurium
as cofactor in the final stage of D-glycerate biosynthesis
(R)-glycerate + NAD+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.60
recombinant His6-tagged GarR from Escherichia coli strain BL21, the tag is removed by TEV protease, purification by nickel affinity chromatography
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.60
tartronate semialdehyde + NADH
as cofactor in the final stage of D-glycerate biosynthesis
699856
Salmonella enterica subsp. enterica serovar Typhimurium
(R)-glycerate + NAD+
-
-
-
?
1.1.1.60
tartronate semialdehyde + NADH + H+
-
699856
Salmonella enterica subsp. enterica serovar Typhimurium
(R)-glycerate + NAD+
-
-
-
?