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Literature summary extracted from

  • Osipiuk, J.; Zhou, M.; Moy, S.; Collart, F.; Joachimiak, A.
    X-ray crystal structure of GarR - tartronate semialdehyde reductase from Salmonella typhimurium (2009), J. Struct. Funct. Genomics, 10, 249-253.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.60 gene garR, expression of the His6-tagged enzyme in Escherichia coli strain BL21 Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.60 enzyme with L-tartrate bound to the active site, sitting drop vapour diffusion method, mixing of 0.0005 ml of the protein solution, containing 107 mg/ml protein in in 10 mM HEPES pH 8.0, 200 mM NaCl, with 0.0005 ml of 0.1 M imidazole buffer pH 8.0, 1 M potassium/sodium L-tartrate and 0.2 M sodium chloride, and equilibrated at 16°C over 0.135 ml of this solution, overnight, X-ray diffraction structure determination and analysis at 1.65 A resolution, the single-wavelength anomalous diffraction method Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.60 tartronate semialdehyde + NADH Salmonella enterica subsp. enterica serovar Typhimurium as cofactor in the final stage of D-glycerate biosynthesis (R)-glycerate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.60 Salmonella enterica subsp. enterica serovar Typhimurium
-
 gene garR
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.60 recombinant His6-tagged GarR from Escherichia coli strain BL21, the tag is removed by TEV protease, purification by nickel affinity chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.60 tartronate semialdehyde + NADH as cofactor in the final stage of D-glycerate biosynthesis Salmonella enterica subsp. enterica serovar Typhimurium (R)-glycerate + NAD+
-
 ?
1.1.1.60 tartronate semialdehyde + NADH + H+
-
 Salmonella enterica subsp. enterica serovar Typhimurium (R)-glycerate + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.60 GarR
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.60 More the enzyme belongs to family of structurally and mechanically related beta-hydroxyacid dehydrogenases, COG2084 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.60 tartronate semialdehyde reductase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.60 TSR
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.60 NADH
-
 Salmonella enterica subsp. enterica serovar Typhimurium