Literature summary extracted from
Oksanen, E.; Blakeley, M.P.; Bonnete, F.; Dauvergne, M.T.; Dauvergne, F.; Budayova-Spano, M.
Large crystal growth by thermal control allows combined X-ray and neutron crystallographic studies to elucidate the protonation states in Aspergillus flavus urate oxidase (2009), J. R. Soc. Interface, 6 Suppl 5, 599-610.
Application
EC Number |
Application |
Comment |
Organism |
---|
1.7.3.3 |
medicine |
urate oxidase is used to reduce toxic urate accumulation during chemotherapy |
Aspergillus flavus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.7.3.3 |
crystals of about a few tens of micrometres in size, which is nucleated previously in crystallization batch containing 5% PEG 8000, 100 mM NaCl, 8 mg/ml uox-substrate complex and 100 mM Tris-HCl pH 8.5, are used as seeds and their size and quality are further improved using a temperature-control device, large crystals of Uox, co-crystallized with its substrates analogues 8-azaxanthine, 9-methyluric acid or the natural substrate in the presence of cyanide (0.5-2 mg/ml), and soaks with the natural substrate in the absence of cyanide, diffracting to high resolutions are obtained, in the presence of different inhibitors, the crystal form of Uox has a body-centred orthorhombic symmetry and one of the largest primitive unit-cell volumes (a: 80 A, b: 96 A, c: 106 A) |
Aspergillus flavus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.7.3.3 |
8-Azaxanthine |
substrate analogue binds in the active site of the enzyme |
Aspergillus flavus |
|
1.7.3.3 |
9-methyluric acid |
substrate analogue |
Aspergillus flavus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.7.3.3 |
137000 |
- |
for the homotetramer, determined by neutron crystallographic analysis |
Aspergillus flavus |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.7.3.3 |
urate + O2 + H2O |
Aspergillus flavus |
purine metabolism |
5-hydroxyisourate + H2O2 |
a metastable intermediate, which is further degrades to allantoin |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.7.3.3 |
Aspergillus flavus |
- |
- |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.7.3.3 |
commercial preparation |
- |
Aspergillus flavus |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.7.3.3 |
urate + O2 + H2O |
purine metabolism |
Aspergillus flavus |
5-hydroxyisourate + H2O2 |
a metastable intermediate, which is further degrades to allantoin |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.7.3.3 |
homotetramer |
determined by X-ray crystallography, formed by crystallographic symmetry operations |
Aspergillus flavus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.7.3.3 |
Uox |
- |
Aspergillus flavus |
1.7.3.3 |
Urate oxidase |
- |
Aspergillus flavus |