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Literature summary extracted from

  • Chen, W.Q.; Graf, C.; Zimmel, D.; Rovina, P.; Krapfenbauer, K.; Jaritz, M.; Parker, P.J.; Lubec, G.; Bornancin, F.
    Ceramide kinase profiling by mass spectrometry reveals a conserved phosphorylation pattern downstream of the catalytic site (2010), J. Proteome Res., 9, 420-429.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.138 expressed in COS-1 cells Homo sapiens
2.7.1.138 recombinant full-length hCERK is obtained from baculovirus-infected Sf9 cells as a glutathione-S-transferase fusion Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.138 S340A by site directed mutagenesis, using of Triton X-100 as lysis buffer results in 15% activity decrease in the mutant protein compared with the wild type enzyme, when octylglucoside is used instead of Triton X-100 for cell lysis, activity is reduced in the S340A mutant protein which reaches only 15% of wild type activity Homo sapiens
2.7.1.138 S340D by site directed mutagenesis, intermediate recovery of 16% is observed for the mutant protein Homo sapiens
2.7.1.138 S427A by site directed mutagenesis, activity in the S427A mutant protein amounts to only 30% of that of wild type enzyme Homo sapiens
2.7.1.138 S427D by site directed mutagenesis, mutant protein displays 50% of wild type activity Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.138 ATP + ceramide Homo sapiens
-
 ADP + ceramide 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.138 Homo sapiens Q8TCT0
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.138 phosphoprotein phosphorylation of serine residues at positions 340 and 408, putative additional phosphorylation site at serine 427 lying in the calcium/calmodulin domain, preventing phosphorylation at S340 results in CERK instability Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.138 by affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.138 ATP + ceramide
-
 Homo sapiens ADP + ceramide 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.138 ceramide kinase
-
 Homo sapiens
2.7.1.138 CERK
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.138 7.2
-
 assay at Homo sapiens