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Literature summary extracted from

  • Doan, T.T.; Carlsson, A.S.; Hamberg, M.; Buelow, L.; Stymne, S.; Olsson, P.
    Functional expression of five Arabidopsis fatty acyl-CoA reductase genes in Escherichia coli (2009), J. Plant Physiol., 166, 787-796.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.42 expressed in Escherichia coli Rosetta (DE3) cells Arabidopsis thaliana
1.2.1.50 into the vector pET15b for expression in Escherichia coli Rosetta cells Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.50 51000
-
 theoretical value, verified by SDS-PAGE Arabidopsis thaliana
1.2.1.50 55500
-
 theoretical value, verified by SDS-PAGE Arabidopsis thaliana
1.2.1.50 56000
-
 theoretical value, verified by SDS-PAGE Arabidopsis thaliana
1.2.1.50 61600
-
 theoretical value, verified by SDS-PAGE Arabidopsis thaliana
1.2.1.50 68400
-
 theoretical value, verified by SDS-PAGE, heavily degraded Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.50 additional information Arabidopsis thaliana catalyses the formation of a fatty alcohol from an acyl-CoA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.42 Arabidopsis thaliana B9TSP7 isozyme FAR6; ecotype Columbia
-
1.2.1.42 Arabidopsis thaliana Q08891 isozyme FAR2; ecotype Columbia
-
1.2.1.42 Arabidopsis thaliana Q39152 isozyme FAR1; ecotype Columbia
-
1.2.1.50 Arabidopsis thaliana B9TSP7
-
-
1.2.1.50 Arabidopsis thaliana Q08891
-
-
1.2.1.50 Arabidopsis thaliana Q1PEI6
-
-
1.2.1.50 Arabidopsis thaliana Q39152
-
-
1.2.1.50 Arabidopsis thaliana Q93ZB9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.50 flower
-
 Arabidopsis thaliana
-
1.2.1.50 root
-
 Arabidopsis thaliana
-
1.2.1.50 stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.42 hexadecanoyl-CoA + NADPH + H+ highest activity Arabidopsis thaliana hexadecanal + CoA + NADP+
-
 ?
1.2.1.42 octadecanoyl-CoA + NADPH + H+
-
 Arabidopsis thaliana octadecanal + CoA + NADP+
-
 ?
1.2.1.42 tetradecanoyl-CoA + NADPH + H+
-
 Arabidopsis thaliana tetradecanal + CoA + NADP+
-
 ?
1.2.1.50 additional information catalyses the formation of a fatty alcohol from an acyl-CoA Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.42 FAR1 isozyme Arabidopsis thaliana
1.2.1.42 FAR2 isozyme Arabidopsis thaliana
1.2.1.42 FAR6 isozyme Arabidopsis thaliana
1.2.1.42 fatty acyl-CoA reductase
-
 Arabidopsis thaliana
1.2.1.50 CER4
-
 Arabidopsis thaliana
1.2.1.50 FAR1
-
 Arabidopsis thaliana
1.2.1.50 FAR2
-
 Arabidopsis thaliana
1.2.1.50 FAR3
-
 Arabidopsis thaliana
1.2.1.50 FAR6
-
 Arabidopsis thaliana
1.2.1.50 FAR8
-
 Arabidopsis thaliana
1.2.1.50 fatty acyl-CoA reductase 1
-
 Arabidopsis thaliana
1.2.1.50 fatty acyl-CoA reductase 2
-
 Arabidopsis thaliana
1.2.1.50 fatty acyl-CoA reductase 3
-
 Arabidopsis thaliana
1.2.1.50 fatty acyl-CoA reductase 6
-
 Arabidopsis thaliana
1.2.1.50 fatty acyl-CoA reductase 8
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.42 NADPH
-
 Arabidopsis thaliana