Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Portes, M.T.; Figueiredo-Ribeiro, R.d.e..C.; de Carvalho, M.A.
    Low temperature and defoliation affect fructan-metabolizing enzymes in different regions of the rhizophores of Vernonia herbacea (2008), J. Plant Physiol., 165, 1572-1581.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.153 additional information Vernonia herbacea hydrolytic cleavage of terminal fructosyl residues off inulin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.99 Vernonia herbacea
-
-
-
3.2.1.153 Vernonia herbacea
-
 18 months old plants, natural outdoor condition (control), excised natural conditions, excised and intact plants at 5°C for 21 days
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.153 precipitated, resuspended, desalted, centrifuged Vernonia herbacea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.99 rhizophore
-
 Vernonia herbacea
-
3.2.1.153 rhizophore
-
 Vernonia herbacea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.153 additional information
-
 highest activities in proximal rhizophores, increased in low temperature and excised plants, and low temperature + excised plants, overall highest activities in low temperature + excised plant rhizophores, overall high fructan content in all treatments, higher fructo-oligosaccharide/fructo-polysaccharide ratios in low temperature treatments, chain size lowest in distal region Vernonia herbacea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.99 sucrose + sucrose
-
 Vernonia herbacea D-glucose + 1-kestose
-
 ?
3.2.1.153 inulin + H2O 5% inulin from Helianthus tuberosus, McIlvaine buffer, pH 4.5, 30°C, 4 h Vernonia herbacea fructose + fructo-oligosaccharide + fructo-polysaccharide
-
 ?
3.2.1.153 additional information hydrolytic cleavage of terminal fructosyl residues off inulin Vernonia herbacea ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.99 1-SST
-
 Vernonia herbacea
2.4.1.99 1-sucrose:sucrose fructosyltransferase
-
 Vernonia herbacea
3.2.1.153 1-FEH
-
 Vernonia herbacea
3.2.1.153 1-fructan exohydrolase
-
 Vernonia herbacea

Expression

EC Number Organism Comment Expression
2.4.1.99 Vernonia herbacea SST-1 is drastically diminished when cold (5°C) and/or excision are imposed down
2.4.1.99 Vernonia herbacea plants at the vegetative stage present high 1-sucrose:sucrose fructosyltransferase in the distal regionof the rhizophores up