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Literature summary extracted from

  • Myers, J.; Ittiprasert, W.; Raghavan, N.; Miller, A.; Knight, M.
    Differences in cysteine protease activity in Schistosoma mansoni-resistant and -susceptible Biomphalaria glabrata and characterization of the hepatopancreas cathepsin B full-length cDNA (2008), J. Parasitol., 94, 659-668.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.1 additional information 3.5fold higher up-regulation of cathepsin B transcript in resistant than in the susceptible snail after Schistosoma mansoni parasite exposure Biomphalaria glabrata

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.1 cloning from a hepatopancreas cDNA library of wild-type strain, adult and Schistosoma mansoni-resistant, BS-90 snails, DNA and amino acid sequence determination and analysis, quantitative expression analysis by real-time RT-PCR Biomphalaria glabrata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.1 E-64
-
 Biomphalaria glabrata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.1 lysosome
-
 Biomphalaria glabrata 5764
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.1 additional information Biomphalaria glabrata differential regulation of snail cathepsin B transcript pre- and postparasite exposure, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Biomphalaria glabrata A7LM75 Schistosoma mansoni-resistant BS-90, or Salvador strain, and Schistosoma mansoni-susceptible M-line strains
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.1 glycoprotein
-
 Biomphalaria glabrata

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.22.1 Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-/- bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides non-active site residue Gln109 and active site Cys115 play an important role in the catalytic mechanism Biomphalaria glabrata
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.1 headfoot anterior region Biomphalaria glabrata
-
3.4.22.1 hepatopancreas posterior region, main activity Biomphalaria glabrata
-
3.4.22.1 ovotestis posterior region Biomphalaria glabrata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.1 additional information
-
 resistant snail BS-90 extract: about 21466 fluorescence units released/min/microgram protein compared with the about 6066 fluorescence units released/min/microgram protein for the susceptible M-line snail extract, overview Biomphalaria glabrata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.1 carbobenzoxy-Phe-Arg-7-amido-4-methylcoumarin + H2O a fluorogenic substrate Biomphalaria glabrata carbobenzoxy-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.1 additional information differential regulation of snail cathepsin B transcript pre- and postparasite exposure, overview Biomphalaria glabrata ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.1 CTSB
-
 Biomphalaria glabrata
3.4.22.1 More cathepsin B of Biomphalaria glabrata belongs to the C1A family of peptidases Biomphalaria glabrata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.1 37
-
 assay at Biomphalaria glabrata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.1 6 6.8 assay at Biomphalaria glabrata