EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.2.5 | additional information | inactive in absence of zinc | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.2.5 | - |
Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.2.5 | D315N | no activity | Escherichia coli |
3.5.2.5 | N94D | no activity | Escherichia coli |
3.5.2.5 | N94V | no activity | Escherichia coli |
3.5.2.5 | R215K | no significant effect on enzyme activity | Escherichia coli |
3.5.2.5 | S288D | no significant effect on enzyme activity | Escherichia coli |
3.5.2.5 | S288V | no significant effect on enzyme activity | Escherichia coli |
3.5.2.5 | S317D | no activity | Escherichia coli |
3.5.2.5 | S317V | no activity | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.2.5 | 28.6 | - |
(S)-allantoin | wild-type allantoinase, supplementation with zinc acetate | Escherichia coli | |
3.5.2.5 | 37.5 | - |
(S)-allantoin | selenomethionine-substituted wild-type allantoinase, no supplementation with zinc acetate | Escherichia coli | |
3.5.2.5 | 53.8 | - |
(S)-allantoin | mutant S288D, supplementation with zinc acetate | Escherichia coli | |
3.5.2.5 | 62.8 | - |
(S)-allantoin | mutant R215K, supplementation with zinc acetate | Escherichia coli | |
3.5.2.5 | 91.3 | - |
(S)-allantoin | mutant S288V, supplementation with zinc acetate | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.2.5 | Zn2+ | required | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.2.5 | (S)-allantoin + H2O | Escherichia coli | final step of ureide pathway | allantoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.2.5 | Escherichia coli | P77671 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.2.5 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.2.5 | (S)-allantoin + H2O | - |
Escherichia coli | allantoate | - |
? | |
3.5.2.5 | (S)-allantoin + H2O | final step of ureide pathway | Escherichia coli | allantoate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.2.5 | homotetramer | - |
Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.2.5 | allantoinase | - |
Escherichia coli |
3.5.2.5 | Fe-allantoinase | - |
Escherichia coli |
3.5.2.5 | metal-dependent allantoinase | - |
Escherichia coli |
3.5.2.5 | zinc-amended allantoinase | - |
Escherichia coli |