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Literature summary extracted from

  • Rubinson, E.H.; Metz, A.H.; OQuin, J.; Eichman, B.F.
    A new protein architecture for processing alkylation damaged DNA: the crystal structure of DNA glycosylase AlkD (2008), J. Mol. Biol., 381, 13-23.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.21 expressed in Escherichia coli HMS174 cells Bacillus cereus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.2.21 sitting drop vapour diffusion method, with 85 mM HEPES (pH 7.5), 15% (w/v) PEG 4000, and 17% (v/v) glycerol, at 21°C Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.21 D113N the mutation results in a 100fold decrease in the single-turnover rate of 7-methylguanine excision relative to the wild type enzyme Bacillus cereus
3.2.2.21 R148A the mutation results in a 100fold decrease in the single-turnover rate of 7-methylguanine excision relative to the wild type enzyme Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.21 Bacillus cereus
-
 strain ATCC 14579
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.21 Ni-NTA column chromatography, heparin affinity column chromatography, and gel filtration Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.21 alkylated DNA + H2O
-
 Bacillus cereus 3-methyladenine + 7-methylguanine + DNA
-
 ?
3.2.2.21 additional information AlkD does not excise 1,N6-ethenoadenine Bacillus cereus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.21 AlkD
-
 Bacillus cereus
3.2.2.21 DNA glycosylase
-
 Bacillus cereus