EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.21 | - |
Oryza sativa |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.21 | free enzyme and its covalent intermediate with 2-deoxy-2-fluoroglucoside at 2.2 A and 1.55 A resolution, respectively. The complex with the 2-fluoroglucoside includes a glycerol molecule, which may make a nucleophilic attack on the anomeric carbon in a transglycosylation reaction. Sugars are positioned as acceptors for transglycosylation by their interactions with E176, the catalytic acid/base, and Y131. Residues I179, N190 and N245 appear to interact with the substrates | Oryza sativa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.21 | I179V | decrease in catalytic efficiencies | Oryza sativa |
3.2.1.21 | L442R | catalytic efficiencies similar to wild-type | Oryza sativa |
3.2.1.21 | N190H | threefold increase in catalytic efficiency towards cellobiose | Oryza sativa |
3.2.1.21 | N245V | twofold increase in catalytic efficiency towards cellobiose | Oryza sativa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.139 | - |
4-nitrophenyl-beta-D-glucopyranoside | mutant N190H, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 0.16 | - |
cellotriose | mutant N190H, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 0.201 | - |
4-nitrophenyl-beta-D-glucopyranoside | mutant L442R, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 0.201 | - |
4-nitrophenyl-beta-D-glucopyranoside | wild-type, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 0.22 | - |
cellotriose | wild-type, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 0.23 | - |
cellotriose | mutant L442R, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 0.95 | - |
4-nitrophenyl-beta-D-glucopyranoside | mutant I179V, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 1.08 | - |
cellotriose | mutant I179V, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 2.1 | - |
4-nitrophenyl-beta-D-glucopyranoside | mutant N245V, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 9.25 | - |
cellotriose | mutant N245V, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 14.3 | - |
cellobiose | mutant N190H, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 21.9 | - |
cellobiose | mutant L442R, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 22 | - |
cellobiose | wild-type, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 61.1 | - |
cellobiose | mutant I179V, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 74.4 | - |
cellobiose | mutant N245V, pH 5.0, 30°C | Oryza sativa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Oryza sativa | Q75I93 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Oryza sativa | 4-nitrophenol + D-glucose | - |
? | |
3.2.1.21 | cellobiose + H2O | - |
Oryza sativa | 2 D-glucose | - |
? | |
3.2.1.21 | cellotriose + H2O | - |
Oryza sativa | D-glucose + cellobiose | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 1.12 | - |
cellobiose | mutant L442R, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 1.16 | - |
cellobiose | wild-type, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 2.09 | - |
cellobiose | mutant N190H, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 2.35 | - |
cellotriose | wild-type, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 2.58 | - |
cellotriose | mutant L442R, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 3.05 | - |
cellotriose | mutant N190H, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 3.3 | - |
cellotriose | mutant I179V, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 3.42 | - |
cellobiose | mutant I179V, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 4.57 | - |
4-nitrophenyl-beta-D-glucopyranoside | mutant L442R, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 4.69 | - |
4-nitrophenyl-beta-D-glucopyranoside | wild-type, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 4.7 | - |
4-nitrophenyl-beta-D-glucopyranoside | mutant N245V, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 6.08 | - |
4-nitrophenyl-beta-D-glucopyranoside | mutant I179V, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 6.58 | - |
cellotriose | mutant N245V, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 6.76 | - |
4-nitrophenyl-beta-D-glucopyranoside | mutant N190H, pH 5.0, 30°C | Oryza sativa | |
3.2.1.21 | 7.17 | - |
cellobiose | mutant N245V, pH 5.0, 30°C | Oryza sativa |