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Literature summary extracted from

  • Fischer, M.; Haase, I.; Kis, K.; Meining, W.; Ladenstein, R.; Cushman, M.; Schramek, N.; Huber, R.; Bacher, A.
    Enzyme catalysis via control of activation entropy: site-directed mutagenesis of 6,7-dimethyl-8-ribityllumazine synthase (2003), J. Mol. Biol., 326, 783-793.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.78
-
 Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.78 A56S kcat is 81.3% of wild-type value Bacillus subtilis
2.5.1.78 D138A kcat is 91.5% of wild-type value Bacillus subtilis
2.5.1.78 E58Q kcat is 70.2% of wild-type value Bacillus subtilis
2.5.1.78 F113S kcat is 6.8% of wild-type value Bacillus subtilis
2.5.1.78 F22D kcat is 14.5% of wild-type value Bacillus subtilis
2.5.1.78 F22S kcat is 47.2% of wild-type value Bacillus subtilis
2.5.1.78 F22V kcat is 26.4% of wild-type value Bacillus subtilis
2.5.1.78 F22W kcat is 43.8% of wild-type value Bacillus subtilis
2.5.1.78 F57S kcat is 43.8% of wild-type value Bacillus subtilis
2.5.1.78 H88A kcat is 12% of wild-type value Bacillus subtilis
2.5.1.78 H88K kcat is 39.5% of wild-type value Bacillus subtilis
2.5.1.78 K131N kcat is 9.7% of wild-type value Bacillus subtilis
2.5.1.78 K131R kcat is 29.8% of wild-type value Bacillus subtilis
2.5.1.78 K135A kcat is 21.9% of wild-type value Bacillus subtilis
2.5.1.78 N23S kcat is 21.9% of wild-type value Bacillus subtilis
2.5.1.78 R127H kcat is 69.7% of wild-type value Bacillus subtilis
2.5.1.78 S142L kcat is 62.3% of wild-type value Bacillus subtilis
2.5.1.78 T80V kcat is 55.1% of wild-type value Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.78 0.0041
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme T80V Bacillus subtilis
2.5.1.78 0.0042
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme A56S Bacillus subtilis
2.5.1.78 0.00612
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme F22W Bacillus subtilis
2.5.1.78 0.00857
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, wild-type enzyme Bacillus subtilis
2.5.1.78 0.01
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme D138A Bacillus subtilis
2.5.1.78 0.0107
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme F22V Bacillus subtilis
2.5.1.78 0.011
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme S142L Bacillus subtilis
2.5.1.78 0.0115
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme K135A Bacillus subtilis
2.5.1.78 0.0119
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme N23S Bacillus subtilis
2.5.1.78 0.0138
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme K131R Bacillus subtilis
2.5.1.78 0.0345
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme F57S Bacillus subtilis
2.5.1.78 0.0355
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme K131N Bacillus subtilis
2.5.1.78 0.042
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme H88K Bacillus subtilis
2.5.1.78 0.042
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme T80V Bacillus subtilis
2.5.1.78 0.05
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme K131R Bacillus subtilis
2.5.1.78 0.0546
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, wild-type enzyme Bacillus subtilis
2.5.1.78 0.056
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme H88K Bacillus subtilis
2.5.1.78 0.0581
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme N23S Bacillus subtilis
2.5.1.78 0.0598
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme F22W Bacillus subtilis
2.5.1.78 0.06
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme H88A Bacillus subtilis
2.5.1.78 0.0636
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme D138A Bacillus subtilis
2.5.1.78 0.0665
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme F113S Bacillus subtilis
2.5.1.78 0.0702
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme A56S Bacillus subtilis
2.5.1.78 0.08
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme F57S Bacillus subtilis
2.5.1.78 0.0849
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme R127H Bacillus subtilis
2.5.1.78 0.0905
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme S142L Bacillus subtilis
2.5.1.78 0.124
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme F22V Bacillus subtilis
2.5.1.78 0.137
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme F22S Bacillus subtilis
2.5.1.78 0.14
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme F22S Bacillus subtilis
2.5.1.78 0.146
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme E58Q Bacillus subtilis
2.5.1.78 0.147
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme H88A Bacillus subtilis
2.5.1.78 0.167
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme K135A Bacillus subtilis
2.5.1.78 0.173
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme E58Q Bacillus subtilis
2.5.1.78 0.278
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme F113S Bacillus subtilis
2.5.1.78 0.283
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme K131N Bacillus subtilis
2.5.1.78 0.675
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme F22D Bacillus subtilis
2.5.1.78 0.72
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme F22D Bacillus subtilis
2.5.1.78 3.14
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme R127H Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.78 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.78
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (3R)-3,4-dihydroxy-2-butanone 4-phosphate
-
 Bacillus subtilis 6,7-dimethyl-8-(1-D-ribityl)lumazine + 2 H2O + phosphate
-
 ?
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate the rate enhancement by the enzyme is predominantly achieved by establishing a favourable topological relation of the two substrates, whereas acid/base catalysis may play a secondary role Bacillus subtilis 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.78 RibH
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.78 37
-
 assay at Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.78 0.0022
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme H88K Bacillus subtilis
2.5.1.78 0.0022
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme H88K Bacillus subtilis
2.5.1.78 0.0038
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme F113S Bacillus subtilis
2.5.1.78 0.0038
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme F113S Bacillus subtilis
2.5.1.78 0.0054
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme K131N Bacillus subtilis
2.5.1.78 0.0054
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme K131N Bacillus subtilis
2.5.1.78 0.0067
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme H88A Bacillus subtilis
2.5.1.78 0.0067
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme H88A Bacillus subtilis
2.5.1.78 0.0081
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme F22D Bacillus subtilis
2.5.1.78 0.0081
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme F22D Bacillus subtilis
2.5.1.78 0.0122
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme K135A Bacillus subtilis
2.5.1.78 0.0122
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme K135A Bacillus subtilis
2.5.1.78 0.0122
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme N23S Bacillus subtilis
2.5.1.78 0.0122
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme N23S Bacillus subtilis
2.5.1.78 0.0147
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme F22V Bacillus subtilis
2.5.1.78 0.0147
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme F22V Bacillus subtilis
2.5.1.78 0.0166
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme K131R Bacillus subtilis
2.5.1.78 0.0166
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme K131R Bacillus subtilis
2.5.1.78 0.0244
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme F22W Bacillus subtilis
2.5.1.78 0.0244
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme F22W Bacillus subtilis
2.5.1.78 0.0244
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme F57S Bacillus subtilis
2.5.1.78 0.0244
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme F57S Bacillus subtilis
2.5.1.78 0.0263
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme F22S Bacillus subtilis
2.5.1.78 0.0263
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme F22S Bacillus subtilis
2.5.1.78 0.0307
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme T80V Bacillus subtilis
2.5.1.78 0.0307
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme T80V Bacillus subtilis
2.5.1.78 0.0347
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme S142L Bacillus subtilis
2.5.1.78 0.0347
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme S142L Bacillus subtilis
2.5.1.78 0.0389
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme R127H Bacillus subtilis
2.5.1.78 0.0389
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme R127H Bacillus subtilis
2.5.1.78 0.0391
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme E58Q Bacillus subtilis
2.5.1.78 0.0391
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme E58Q Bacillus subtilis
2.5.1.78 0.0453
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme A56S Bacillus subtilis
2.5.1.78 0.0453
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme A56S Bacillus subtilis
2.5.1.78 0.051
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, mutant enzyme D138A Bacillus subtilis
2.5.1.78 0.051
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, mutant enzyme D138A Bacillus subtilis
2.5.1.78 0.0557
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione pH 7.0, 37°C, wild-type enzyme Bacillus subtilis
2.5.1.78 0.0557
-
 L-3,4-dihydroxybutan-2-one 4-phosphate pH 7.0, 37°C, wild-type enzyme Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.78 7
-
 assay at Bacillus subtilis