Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jeon, Y.M.; Kim, B.G.; Ahn, J.H.
    Biological synthesis of 7-O-methyl Apigenin from naringenin using escherichia coli expressing two genes (2009), J. Microbiol. Biotechnol., 19, 491-494.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.20.5 biotechnology product of the combined activity of flavone synthase and flavone 7-O-methyltransferase produces genkwanin (7-O-methyl apigenin) which exhibits antibacterial activity against Vibrio cholerae and Enterococcus faecalis, and anti-inflammatory activity, direct plant extracts result in limited amounts Populus deltoides
2.1.1.B76 synthesis using Escherichia coli transformants harboring both PFNS-1 and POMT-7, naringenin could be converted into genkwanin Populus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.20.5 the open reading frame of the flavone synthase gene PFNS-1 is cloned from Populus deltoids using RT-PCR, subcloned into Escherichia coli expression vector pGEX 5X-2, the flavone 7-O-methyltransferase into pRSF-Duet vector, both cotransformed into Escherichia coli BL21 Populus deltoides
2.1.1.B74 gene POMT-7, recombinant expression of His-tagged enzyme in Escherichia coli Populus deltoides
2.1.1.B76 using Escherichia coli transformants harboring both PFNS-1 and POMT-7, naringenin could be converted into genkwanin Populus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.20.5 64000
-
 SDS-PAGE, sum of flavone synthase and glutathione S-transferase (26000 Da) Populus deltoides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.20.5 naringenin + O2 + 2-oxoglutarate Populus deltoides
-
 apigenin + succinate + CO2 + H2O flavone 7-O-methyltransferase (POMT-7) converts apigenin into 7-O-methyl apigenin (genkwanin), 28°C, 10 mM potassium phosphate buffer with 1% glucose, pH 6.0, 5 mM ascorbate, 10 microM FeSO4, 100 microM substrate naringenin, 15 h incubation ?
2.1.1.B74 S-adenosyl-L-methionine + apigenin Populus deltoides
-
 S-adenosyl-L-homocysteine + 7-O-methylapigenin i.e. genkwanin ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.5 Populus deltoides
-
-
-
2.1.1.B74 Populus deltoides
-
 gene POMT-7
-
2.1.1.B76 Populus sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.5 naringenin + O2 + 2-oxoglutarate
-
 Populus deltoides apigenin + succinate + CO2 + H2O flavone 7-O-methyltransferase (POMT-7) converts apigenin into 7-O-methyl apigenin (genkwanin), 28°C, 10 mM potassium phosphate buffer with 1% glucose, pH 6.0, 5 mM ascorbate, 10 microM FeSO4, 100 microM substrate naringenin, 15 h incubation ?
2.1.1.B74 S-adenosyl-L-methionine + apigenin
-
 Populus deltoides S-adenosyl-L-homocysteine + 7-O-methylapigenin i.e. genkwanin ?
2.1.1.B76 S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone i.e. apigenin Populus sp. S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone i.e. genkwanin ?

Synonyms

EC Number Synonyms Comment Organism
1.14.20.5 flavone synthase
-
 Populus deltoides
1.14.20.5 PFNS-1
-
 Populus deltoides
2.1.1.B74 flavone 7-O-methyltransferase
-
 Populus deltoides
2.1.1.B74 POMT-7
-
 Populus deltoides
2.1.1.B76 POMT-7
-
 Populus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.B74 28
-
 assay at Populus deltoides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.20.5 6
-
 optimum for the combined action of both enzymes, next optimal pH is 8.0, then pH 7.0 Populus deltoides
2.1.1.B74 6
-
 recombinant enzyme Populus deltoides

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.B74 S-adenosyl-L-methionine
-
 Populus deltoides