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Literature summary extracted from
- Purification and characterization of an extracellular beta-glucosidase from Monascus purpureus (2008), J. Microbiol. Biotechnol., 18, 933-941.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | ethanol | slight stimulation at low concentrations | Monascus purpureus |  |
| 3.2.1.21 | methanol | slight stimulation at low concentrations | Monascus purpureus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | D-glucose | 10 mM, 42% residual activity | Monascus purpureus | |
| 3.2.1.21 | HgCl2 | 5 mM, 14% residual activity | Monascus purpureus | |
| 3.2.1.21 | K2Cr2O7 | 5 mM, 49% residual activity | Monascus purpureus | |
| 3.2.1.21 | K2CrO4 | 5 mM, 80% residual activity | Monascus purpureus | |
| 3.2.1.21 | additional information | not inhibitory: 2-mercaptoethanol, sodium dodecyl sulfate, EDTA | Monascus purpureus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Monascus purpureus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | - |
Monascus purpureus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 84.3 | - |
pH 4.5, 37°C | Monascus purpureus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.21 | -15°C, 60 days, no significant loss in activity | Monascus purpureus |
| 3.2.1.21 | 5°C, 60 days, no significant loss in activity | Monascus purpureus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-nitrophenyl beta-D-cellobioside + 2 H2O | - |
Monascus purpureus | 4-nitrophenol + 2 D-glucose | glucosyl residues are removed from the non-reducing end of substrate | ? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Monascus purpureus | 4-nitrophenol + D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Monascus purpureus | 2 D-glucose | - |
? | |
| 3.2.1.21 | maltose + H2O | at 125% of the rate with cellobiose | Monascus purpureus | 2 D-glucose | - |
? | |
| 3.2.1.21 | n-octyl-beta-D-glucopyranoside + H2O | at 75% of the rate with cellobiose | Monascus purpureus | n-octanol + D-glucose | - |
? | |
| 3.2.1.21 | salicin + H2O | at 70% of the rate with cellobiose | Monascus purpureus | salicyl alcohol + D-glucose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
- |
Monascus purpureus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
2 h, completely stable | Monascus purpureus |
| 3.2.1.21 | 60 | - |
1 h, 83% residual activity | Monascus purpureus |
| 3.2.1.21 | 70 | - |
half-life 1.8 min | Monascus purpureus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5.5 | - |
- |
Monascus purpureus |
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Release 2023.1 (January 2023)
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