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Literature summary extracted from

  • Goldbaum, F.A.; Velikovsky, C.A.; Baldi, P.C.; Mörtl, S.; Bacher, A.; Fossati, C.A.
    The 18-kDa cytoplasmic protein of Brucella species - an antigen useful for diagnosis - is a lumazine synthase (1999), J. Med. Microbiol., 48, 833-839.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.78 medicine this protein constitutes an interesting candidate for serological diagnosis and for the design of specific chemotherapeutic agents, and its polymeric characteristics could provide the basis for the development of an acellular vaccine Brucella sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.78 expression in Escherichia coli. The recombinant protein is soluble only under reducing conditions, but alkylation with iodoacetamide renders it soluble in non-reducing media Brucella sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.78 cytoplasm
-
 Brucella sp. 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.78 18000
-
 x * 18000, SDS-PAGE Brucella sp.
2.5.1.78 90000
-
 gel filtration Brucella sp.

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.78 Brucella sp. P61711
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.78
-
 Brucella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Brucella sp. 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.78 ? x * 18000, SDS-PAGE Brucella sp.
2.5.1.78 More Brucella spp. lumazine synthase arranges in icosahedric capsids similar to those formed by the lumazine synthases of other bacteria Brucella sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.78 37
-
 assay at Brucella sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.78 7
-
 assay at Brucella sp.