BRENDA - Enzyme Database show

Cloning, expression, post-Translational modifications and inhibition Studies on the latest mammalian carbonic anhydrase isoform, CA XV

Hilvo, M.; Salzano, A.M.; Innocenti, A.; Kulomaa, M.S.; Scozzafava, A.; Scaloni, A.; Parkkila, S.; Supuran, C.T.; J. Med. Chem. 52, 646-654 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.1
expressed in a baculovirus-Sf9 insect cell expression system
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
2-aminobenzenesulfonamide
-
Mus musculus
4.2.1.1
2-hydrazinobenzenesulfonamide
-
Mus musculus
4.2.1.1
4-(2-aminoethyl)benzenesulfonamide
-
Mus musculus
4.2.1.1
4-(2-hydroxyethyl)benzenesulfonamide
-
Mus musculus
4.2.1.1
4-(aminomethyl)benzenesulfonamide
-
Mus musculus
4.2.1.1
4-(hydroxymethyl)benzenesulfonamide
-
Mus musculus
4.2.1.1
4-amino-3-bromobenzenesulfonamide
-
Mus musculus
4.2.1.1
4-amino-3-chlorobenzenesulfonamide
-
Mus musculus
4.2.1.1
4-amino-3-fluorobenzenesulfonamide
-
Mus musculus
4.2.1.1
4-amino-3-iodobenzenesulfonamide
-
Mus musculus
4.2.1.1
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
Mus musculus
4.2.1.1
4-amino-6-chlorobenzene-1,3-disulfonamide
-
Mus musculus
4.2.1.1
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
Mus musculus
4.2.1.1
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
Mus musculus
4.2.1.1
4-Aminobenzenesulfonamide
-
Mus musculus
4.2.1.1
4-hydrazinobenzenesulfonamide
-
Mus musculus
4.2.1.1
4-methylbenzenesulfonamide
-
Mus musculus
4.2.1.1
4-sulfamoylbenzoic acid
-
Mus musculus
4.2.1.1
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
Mus musculus
4.2.1.1
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
-
Mus musculus
4.2.1.1
5-amino-1,3,4-thiadiazole-2-sulfonamide
-
Mus musculus
4.2.1.1
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
Mus musculus
4.2.1.1
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
-
Mus musculus
4.2.1.1
6-hydroxy-1,3-benzothiazole-2-sulfonamide
-
Mus musculus
4.2.1.1
acetazolamide
-
Mus musculus
4.2.1.1
benzolamide
-
Mus musculus
4.2.1.1
brinzolamide
best isozyme CA XV inhibitor
Mus musculus
4.2.1.1
celecoxib
best isozyme CA XV inhibitor
Mus musculus
4.2.1.1
dichlorophenamide
-
Mus musculus
4.2.1.1
dorzolamide
best isozyme CA XV inhibitor
Mus musculus
4.2.1.1
ethoxzolamide
best isozyme CA XV inhibitor
Mus musculus
4.2.1.1
indisulam
-
Mus musculus
4.2.1.1
methazolamide
best isozyme CA XV inhibitor
Mus musculus
4.2.1.1
sulpiride
-
Mus musculus
4.2.1.1
sulthiame
best isozyme CA XV inhibitor
Mus musculus
4.2.1.1
topiramate
-
Mus musculus
4.2.1.1
valdecoxib
-
Mus musculus
4.2.1.1
zonisamide
-
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.1
470000
-
CO2
at 20C and pH 7.5
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.1
membrane
-
Mus musculus
16020
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.1
Mus musculus
Q99N23
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.1
-
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
CO2 + H2O
-
699472
Mus musculus
H2CO3
-
-
-
r
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.1
14.2
-
CO2
at 20C and pH 7.5
Mus musculus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.1
0.000045
-
celecoxib
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000048
-
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000048
-
5-amino-1,3,4-thiadiazole-2-sulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000049
-
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000049
-
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000058
-
ethoxzolamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00006
-
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000061
-
brinzolamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000061
-
dorzolamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000062
-
4-amino-3-chlorobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000063
-
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000064
-
6-hydroxy-1,3-benzothiazole-2-sulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000065
-
methazolamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000065
-
sulthiame
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000066
-
valdecoxib
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000067
-
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00007
-
benzolamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000072
-
4-amino-3-iodobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000072
-
acetazolamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000072
-
indisulam
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000073
-
sulpiride
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000078
-
topiramate
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000079
-
4-amino-3-fluorobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000083
-
4-amino-3-bromobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000095
-
dichlorophenamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000634
-
zonisamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000757
-
4-amino-6-chlorobenzene-1,3-disulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000783
-
4-(2-aminoethyl)benzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000926
-
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00097
-
4-(aminomethyl)benzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00456
-
4-(hydroxymethyl)benzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00465
-
4-sulfamoylbenzoic acid
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00466
-
4-(2-hydroxyethyl)benzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.006895
-
2-hydrazinobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00799
-
4-methylbenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.008805
-
2-aminobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00954
-
4-hydrazinobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00961
-
4-Aminobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.1
expressed in a baculovirus-Sf9 insect cell expression system
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
2-aminobenzenesulfonamide
-
Mus musculus
4.2.1.1
2-hydrazinobenzenesulfonamide
-
Mus musculus
4.2.1.1
4-(2-aminoethyl)benzenesulfonamide
-
Mus musculus
4.2.1.1
4-(2-hydroxyethyl)benzenesulfonamide
-
Mus musculus
4.2.1.1
4-(aminomethyl)benzenesulfonamide
-
Mus musculus
4.2.1.1
4-(hydroxymethyl)benzenesulfonamide
-
Mus musculus
4.2.1.1
4-amino-3-bromobenzenesulfonamide
-
Mus musculus
4.2.1.1
4-amino-3-chlorobenzenesulfonamide
-
Mus musculus
4.2.1.1
4-amino-3-fluorobenzenesulfonamide
-
Mus musculus
4.2.1.1
4-amino-3-iodobenzenesulfonamide
-
Mus musculus
4.2.1.1
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
Mus musculus
4.2.1.1
4-amino-6-chlorobenzene-1,3-disulfonamide
-
Mus musculus
4.2.1.1
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
Mus musculus
4.2.1.1
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
Mus musculus
4.2.1.1
4-Aminobenzenesulfonamide
-
Mus musculus
4.2.1.1
4-hydrazinobenzenesulfonamide
-
Mus musculus
4.2.1.1
4-methylbenzenesulfonamide
-
Mus musculus
4.2.1.1
4-sulfamoylbenzoic acid
-
Mus musculus
4.2.1.1
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
Mus musculus
4.2.1.1
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
-
Mus musculus
4.2.1.1
5-amino-1,3,4-thiadiazole-2-sulfonamide
-
Mus musculus
4.2.1.1
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
Mus musculus
4.2.1.1
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
-
Mus musculus
4.2.1.1
6-hydroxy-1,3-benzothiazole-2-sulfonamide
-
Mus musculus
4.2.1.1
acetazolamide
-
Mus musculus
4.2.1.1
benzolamide
-
Mus musculus
4.2.1.1
brinzolamide
best isozyme CA XV inhibitor
Mus musculus
4.2.1.1
celecoxib
best isozyme CA XV inhibitor
Mus musculus
4.2.1.1
dichlorophenamide
-
Mus musculus
4.2.1.1
dorzolamide
best isozyme CA XV inhibitor
Mus musculus
4.2.1.1
ethoxzolamide
best isozyme CA XV inhibitor
Mus musculus
4.2.1.1
indisulam
-
Mus musculus
4.2.1.1
methazolamide
best isozyme CA XV inhibitor
Mus musculus
4.2.1.1
sulpiride
-
Mus musculus
4.2.1.1
sulthiame
best isozyme CA XV inhibitor
Mus musculus
4.2.1.1
topiramate
-
Mus musculus
4.2.1.1
valdecoxib
-
Mus musculus
4.2.1.1
zonisamide
-
Mus musculus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.1
0.000045
-
celecoxib
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000048
-
5-[[(4-aminophenyl)sulfonyl]amino]-1,3,4-thiadiazole-2-sulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000048
-
5-amino-1,3,4-thiadiazole-2-sulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000049
-
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000049
-
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000058
-
ethoxzolamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00006
-
5-(2-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000061
-
brinzolamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000061
-
dorzolamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000062
-
4-amino-3-chlorobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000063
-
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000064
-
6-hydroxy-1,3-benzothiazole-2-sulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000065
-
methazolamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000065
-
sulthiame
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000066
-
valdecoxib
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000067
-
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00007
-
benzolamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000072
-
4-amino-3-iodobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000072
-
acetazolamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000072
-
indisulam
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000073
-
sulpiride
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000078
-
topiramate
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000079
-
4-amino-3-fluorobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000083
-
4-amino-3-bromobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000095
-
dichlorophenamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000634
-
zonisamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000757
-
4-amino-6-chlorobenzene-1,3-disulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000783
-
4-(2-aminoethyl)benzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.000926
-
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00097
-
4-(aminomethyl)benzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00456
-
4-(hydroxymethyl)benzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00465
-
4-sulfamoylbenzoic acid
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00466
-
4-(2-hydroxyethyl)benzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.006895
-
2-hydrazinobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00799
-
4-methylbenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.008805
-
2-aminobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00954
-
4-hydrazinobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
4.2.1.1
0.00961
-
4-Aminobenzenesulfonamide
at 20C and pH 7.5
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.1
470000
-
CO2
at 20C and pH 7.5
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.1
membrane
-
Mus musculus
16020
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.1
-
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
CO2 + H2O
-
699472
Mus musculus
H2CO3
-
-
-
r
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.1
14.2
-
CO2
at 20C and pH 7.5
Mus musculus