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Literature summary extracted from

  • Fujita, K.; Chan, J.M.; Bollinger, J.A.; Alvarez, M.L.; Dooley, D.M.
    Anaerobic purification, characterization and preliminary mechanistic study of recombinant nitrous oxide reductase from Achromobacter cycloclastes (2007), J. Inorg. Biochem., 101, 1836-1844.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.4 the gene encoding the enzyme in the nos cluster is cloned from a genomic library Achromobacter cycloclastes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.4 additional information
-
 additional information steady-state kinetics Achromobacter cycloclastes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.4 Cu2+ the enzyme contains average 4.5 Cu and 1.2 S per monomer Achromobacter cycloclastes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.4 nitrous oxide + reduced methyl viologen Achromobacter cycloclastes
-
 nitrogen + H2O + methyl viologen
-
 ?
1.7.2.4 nitrous oxide + reduced methyl viologen Achromobacter cycloclastes IAM1013
-
 nitrogen + H2O + methyl viologen
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.4 Achromobacter cycloclastes P94127
-
-
1.7.2.4 Achromobacter cycloclastes IAM1013 P94127
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.4 anaerobical purification of N2OR under an Ar atmosphere by three different steps of anion exchange chromatography, followed by cytochrome c affinity chromatography and ultrafiltration Achromobacter cycloclastes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.4 124
-
 purified enzyme, pH 8.0, 25°C Achromobacter cycloclastes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.4 nitrous oxide + reduced methyl viologen
-
 Achromobacter cycloclastes nitrogen + H2O + methyl viologen
-
 ?
1.7.2.4 nitrous oxide + reduced methyl viologen
-
 Achromobacter cycloclastes IAM1013 nitrogen + H2O + methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.4 More perturbations of the protein conformation induced by pH variations, although the principal secondary structure elements are largely unaltered Achromobacter cycloclastes

Synonyms

EC Number Synonyms Comment Organism
1.7.2.4 N2OR
-
 Achromobacter cycloclastes
1.7.2.4 nitrous oxide reductase
-
 Achromobacter cycloclastes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.2.4 25
-
 assay at Achromobacter cycloclastes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.4 7.1 8 assay at Achromobacter cycloclastes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.2.4 5.7 10.6 perturbations of the protein conformation induced by pH variations, although the principal secondary structure elements are largely unaltered Achromobacter cycloclastes