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Literature summary extracted from
- Predicting enzyme behavior in nonconventional media: correlating nitrilase function with solvent properties (2008), J. Ind. Microbiol. Biotechnol., 35, 713-720.
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.5.5.1 | Acetone | extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-15% | Alcaligenes faecalis |
| 3.5.5.1 | dimethyl acetamide | extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced at 5% | Alcaligenes faecalis |
| 3.5.5.1 | dimethyl formamide | extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-25% | Alcaligenes faecalis |
| 3.5.5.1 | dimethyl sulfoxide | signifantly enhances the synthetic potential of the enzyme as well as its enantioselectivity. Extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 525% | Alcaligenes faecalis |
| 3.5.5.1 | dioxane | extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-10% | Alcaligenes faecalis |
| 3.5.5.1 | Ethanol | extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-20% | Alcaligenes faecalis |
| 3.5.5.1 | isopropanol | extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-15% | Alcaligenes faecalis |
| 3.5.5.1 | Methanol | extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-20% | Alcaligenes faecalis |
| 3.5.5.1 | additional information | addition of organic solvent up to 10%-20% leads to an enhancement in reaction rate, any further increase beyond the critical concentration in the latter leads to the decrease in catalytic efficiency of the enzyme. The solvent dielectric constant shows a linear correlation with the critical concentration of the solvent used and the extent of nitrile hydrolysis. Unlike alcohols, the reaction rate in case of aprotic solvents can be linearly correlated to solvent log P. The affinity of the enzyme for its substrate is highly dependent upon the aprotic solvent used | Alcaligenes faecalis |
| 3.5.5.1 | N-methyl pyrrolidone | addition results in decreased rate of hydrolysis | Alcaligenes faecalis |
| 3.5.5.1 | n-propanol | extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-15% | Alcaligenes faecalis |
| 3.5.5.1 | N-vinyl pyrrolidone | addition results in decreased rate of hydrolysis | Alcaligenes faecalis |
| 3.5.5.1 | Pyridine | addition results in decreased rate of hydrolysis | Alcaligenes faecalis |
| 3.5.5.1 | tetrahydrofuran | addition results in decreased rate of hydrolysis | Alcaligenes faecalis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.5.1 | Alcaligenes faecalis | - |
- |
- |
| 3.5.5.1 | Alcaligenes faecalis MTCC 126 | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.1 | 2 (R,S)-mandelonitrile + 4 H2O | - |
Alcaligenes faecalis | (R)-mandelic acid + NH3 + (S)-mandelonitrile | - |
? |  |
| 3.5.5.1 | 2 (R,S)-mandelonitrile + 4 H2O | - |
Alcaligenes faecalis MTCC 126 | (R)-mandelic acid + NH3 + (S)-mandelonitrile | - |
? | |
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Release 2023.1 (January 2023)
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