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Literature summary extracted from

  • Kucerova, L.; Matuskova, M.; Pastorakova, A.; Tyciakova, S.; Jakubikova, J.; Bohovic, R.; Altanerova, V.; Altaner, C.
    Cytosine deaminase expressing human mesenchymal stem cells mediated tumour regression in melanoma bearing mice (2008), J. Gene Med., 10, 1071-1082.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.4.1 pharmacology applicability of gene-directed enzyme prodrug therapy using the capability of human adipose tissue-derived mesenchymal stem cells (AT-MSC) as cellular vehicles expressing cytosine deaminase, CDy-AT-MSC/5FC-system, analyzed in cell lines and xenografts Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.1 stable transduction of human mesenchymal stem cells derived from adipose tissue (AT-MAS) with retroviral pST2 vector bearing yeast fusion cytosine deaminase, GP+E-86 cells and GP+envAM12 used as retroviral packaging mouse cell line, NIH-3T3 mouse fibroblast cells used for titration of viral inoculum Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.1 cytosine + H2O Saccharomyces cerevisiae
-
 uracil + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.1 Saccharomyces cerevisiae Q12178 recombinant protein
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.1 additional information
-
 capability of human adipose tissue-derived mesenchymal stem cells (AT-MSC) as vehicles for gene-directed enzyme prodrug therapy, fusion product of cytosine deaminase derived from yeast in combination with systemic 5-fluorocytosine (5-Fc), expression and antitumoral effects of fusion constructs tested in cell lines and xenograft models, in vivo analysis in immunodeficient mouse model, regression in 89% of tumors achieved, therapeutic cell homing into subcutaneous melanoma and inhibition of tumor growth Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.1 5-fluorocytosine + H2O recombinant cytosine deaminase in gene therapy, expression and function in transgenic cell lines and xenograft tumors Saccharomyces cerevisiae 5-fluorouracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Saccharomyces cerevisiae uracil + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.1 CD
-
 Saccharomyces cerevisiae