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Literature summary extracted from

  • Kaessler, A.; Nourrisson, M.R.; Duflos, M.; Jose, J.
    Indole carboxamides inhibit bovine testes hyaluronidase at pH 7.0 and indole acetamides activate the enzyme at pH 3.5 by different mechanisms (2008), J. Enzyme Inhib. Med. Chem., 23, 719-727.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.35 bovine serum albumine
-
 Bos taurus
3.2.1.35 N-(4,6-dimethylpyridin-2-yl)-(1-ethylindole-3-yl)acetamide acts as an inhibitor at pH 7 and at pH 3.5 activity is increased to 134% Bos taurus
3.2.1.35 N-(4,6-dimethylpyridin-2-yl)-[5-bromo-1-(4-methyl)indole-3-yl]carboxamide acts as an inhibitor at pH 7 and at pH 3.5 activity is increased to 117% Bos taurus
3.2.1.35 N-(pyridin-4yl)-[5-bromo-1-(4-fluorobenzyl)indole-3-yl]carboxamide acts as an inhibitor at pH 7 and at pH 3.5 activity is increased to 120% Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.35 ascorbic acid palmitate inhibition at pH 7: 99%, at pH 3.5: 99% Bos taurus
3.2.1.35 N-(4,6-dimethylpyridin-2-yl)-(1-ethylindole-3-yl)acetamide inhibition at pH 7: 3%, but at pH 3.5: 134% activation Bos taurus
3.2.1.35 N-(4,6-dimethylpyridin-2-yl)-[5-bromo-1-(4-methyl)indole-3-yl]carboxamide inhibition at pH 7: 37%, but at pH 3.5: 117% activation Bos taurus
3.2.1.35 N-(pyridin-4yl)-[5-bromo-1-(4-fluorobenzyl)indole-3-yl]carboxamide inhibition at pH 7: 50%, but at pH 3.5: 120% activation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 testis
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 hyaluronic acid + H2O
-
 Bos taurus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 hyaluronidase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.35 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 7
-
 assay at Bos taurus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.35 0.008
-
 pH: 7.0 Bos taurus ascorbic acid palmitate
3.2.1.35 0.018
-
 pH: 3.5 Bos taurus ascorbic acid palmitate
3.2.1.35 0.046
-
 pH: 7.0 Bos taurus N-(pyridin-4yl)-[5-bromo-1-(4-fluorobenzyl)indole-3-yl]carboxamide
3.2.1.35 0.078
-
 pH: 7.0 Bos taurus N-(4,6-dimethylpyridin-2-yl)-[5-bromo-1-(4-methyl)indole-3-yl]carboxamide