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Literature summary extracted from
- Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation (2009), J. Biotechnol., 139, 203-210.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.9 | gene ilvD, expression in Escherichia coli DH5alpha, overexpression | Corynebacterium glutamicum |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.1.9 | additional information | construction of a valine producer by genetic engineering, mutant strains are resistant against inhibition by branched-chain amino acids L-valine, L-isoleucine and L-leucine, A constructed weak promoter of ilvA or leuA, which is introduced into the Corynebacterium glutamicum chromosome via a gene-replacement technique, reduces the biosynthetic rate of isoleucine or leucine, which lowers the mutant growth rate and increases valine production. Overexpression of ilvD driven by the strong mutant promoter P-ilvDM7 resultsin an even higher level of valine production, engineered strains, overview | Corynebacterium glutamicum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.9 | 2,3-dihydroxy-3-methylvalerate | Corynebacterium glutamicum | - |
3-methyl-2-oxovalerate + H2O | - |
? |  |
| 4.2.1.9 | 2,3-dihydroxy-isovalerate | Corynebacterium glutamicum | - |
2-oxoisovalerate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.9 | Corynebacterium glutamicum | - |
gene ilvD, strain ilvNM13, ATCC 13032 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.9 | 2,3-dihydroxy-3-methylvalerate | - |
Corynebacterium glutamicum | 3-methyl-2-oxovalerate + H2O | - |
? | |
| 4.2.1.9 | 2,3-dihydroxy-isovalerate | - |
Corynebacterium glutamicum | 2-oxoisovalerate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.9 | DHAD | - |
Corynebacterium glutamicum |
| 4.2.1.9 | dihydroxyacid dehydratase | - |
Corynebacterium glutamicum |
| 4.2.1.9 | IlvD | - |
Corynebacterium glutamicum |
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Release 2023.1 (January 2023)
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