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Literature summary extracted from

  • Waheed, A.; Hassan, M.I.; Etten, R.L.; Ahmad, F.
    Human seminal proteinase and prostate-specific antigen are the same protein (2008), J. Biosci., 33, 195-207.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.77 additional information human seminal proteinase and prostate-specific antigen are identical Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.77 EDTA 10 mM inhibits at pH 3.5 Homo sapiens
3.4.21.77 iodoacetate 3 mM inhibits at pH 7.5 with casein as a substrate and at pH 3.5 with bovine serum albumin as a substrate Homo sapiens
3.4.21.77 leupeptin 1 mM completely inhibits with bovine serum albumin as a substrate at pH 3.5 Homo sapiens
3.4.21.77 methylmethane thiosulphonate 1 mM completely inhibits with bovine serum albumin as a substrate at pH 3.5 Homo sapiens
3.4.21.77 additional information 5 mM Ca2+ or p-mercaptoethanol do not inhibit Homo sapiens
3.4.21.77 N-p-tosyl-Lys chloromethyl ketone 3 mM inhibits at pH 3.5 with bovine serum albumin as a substrate but does not inhibit enzyme activity at pH 7.5 with casein as a substrate Homo sapiens
3.4.21.77 N-tosyl-Phe chloromethyl ketone 3 mM inhibits at pH 3.5 with bovine serum albumin as a substrate but does not inhibit enzyme activity at pH 7.5 with casein as a substrate Homo sapiens
3.4.21.77 p-hydroxyphenylmercurisulfonate 1 mM completely inhibits with bovine serum albumin as a substrate at pH 3.5 Homo sapiens
3.4.21.77 Phenylmethylsulphonyl fluoride 3 mM inhibits at pH 7.5 with casein as a substrate and at pH 3.5 with bovine serum albumin as a substrate Homo sapiens
3.4.21.77 Zn2+ 3 mM inhibits at pH 7.5 with casein as a substrate. When bovine serum albumin is used as a substrate at pH 3.5, prostate-specific antigen or proteinase is not inhibited by 5 mM Zn2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.77 30000
-
 calculated from the amino acid composition of isolated prostate-specific antigen and proteinase Homo sapiens
3.4.21.77 34000
-
 calculated from the elution profile Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.77 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.77 by centrifugation, fractional precipitation by ammonium sulphate, ion-exchange chromatography and gel filtration, purified to homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.77 additional information seminal fluid Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.4.21.77 4°C, Tris-HCl buffer Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.77 Bovine serum albumin + H2O is more readily hydrolysed than casein Homo sapiens ?
-
 ?
3.4.21.77 casein + H2O proteolytic activity at pH 7.5 Homo sapiens ?
-
 ?
3.4.21.77 Gelatin + H2O proteolytic activity at pH 7.5 Homo sapiens ?
-
 ?
3.4.21.77 N-succinyl-(Ala)3-p-nitroanilide + H2O prostate-specific antigen shows limited activity Homo sapiens ?
-
 ?
3.4.21.77 N-succinyl-Ala-Ala-Pro-Phe-Ala-p-nitroanilide + H2O prostate-specific antigen shows limited activity Homo sapiens ?
-
 ?
3.4.21.77 N-succinyl-Ala-Ala-Pro-Val-p-nitroanilide + H2O prostate-specific antigen shows limited activity Homo sapiens ?
-
 ?
3.4.21.77 N-succinyl-Gly-Pro-Lys-p-nitroanilide + H2O prostate-specific antigen activity is 3times higher towards this substrate than towards other synthetic substrates Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.77 prostate-specific antigen
-
 Homo sapiens
3.4.21.77 PSA
-
 Homo sapiens
3.4.21.77 seminal proteinase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.77 3.5
-
 bovine serum albumin is degraded 3-4times faster at pH 3.5 than at pH 7.5 Homo sapiens