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Literature summary extracted from

  • Lee, J.W.; Park, J.Y.; Kwon, M.; Choi, I.G.
    Purification and characterization of a thermostable xylanase from the brown-rot fungus Laetiporus sulphureus (2009), J. Biosci. Bioeng., 107, 33-37.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Co2+ in the presence of 10 mM, the relative xylanase activity decreases by 5% Laetiporus sulphureus
3.2.1.8 Cu2+ in the presence of 10 mM, the relative xylanase activity decreases by 58.1% Laetiporus sulphureus
3.2.1.8 EDTA in the presence of 10 mM, the relative xylanase activity decreases by 12.6% Laetiporus sulphureus
3.2.1.8 Fe2+ in the presence of 10 mM, the relative xylanase activity decreases by 21.1% Laetiporus sulphureus
3.2.1.8 Mg2+ in the presence of 10 mM, the relative xylanase activity decreases by 19.1% Laetiporus sulphureus
3.2.1.8 Mn2+ in the presence of 10 mM, the relative xylanase activity decreases by 44.3% Laetiporus sulphureus
3.2.1.8 Zn2+ in the presence of 10 mM, the relative xylanase activity decreases by 9.2% Laetiporus sulphureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Ca2+ in the presence of 5 mM, the relative xylanase activity increases by 35.9% Laetiporus sulphureus
3.2.1.8 potassium acetate in the presence of 5 mM, the relative xylanase activity increases by 5.8% Laetiporus sulphureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 69300
-
 1 * 70000, SDS-PAGE, 1 * 69300, LabChip microfluidic system Laetiporus sulphureus
3.2.1.8 70000
-
 1 * 70000, SDS-PAGE, 1 * 69300, LabChip microfluidic system Laetiporus sulphureus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Laetiporus sulphureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 three consecutive purification steps, gel filtration, 96% purity Laetiporus sulphureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 4.9
-
 crude filtrate Laetiporus sulphureus
3.2.1.8 72.4
-
 purified enzyme Laetiporus sulphureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 avicel + H2O 10% activity as compared to beechwood xylan Laetiporus sulphureus ?
-
 ?
3.2.1.8 beechwood xylan + H2O highest activity Laetiporus sulphureus ?
-
 ?
3.2.1.8 birchwood xylan + H2O slightly lower activity than with beechwood xylan Laetiporus sulphureus xylobiose + xylotetraose + ?
-
 ?
3.2.1.8 carboxylmethyl cellulose + H2O 10% activity as compared to beechwood xylan Laetiporus sulphureus ?
-
 ?
3.2.1.8 additional information does not hydrolyze xylobiose Laetiporus sulphureus ?
-
 ?
3.2.1.8 oat spelt xylan + H2O 22.3% activity as compared to beechwood xylan Laetiporus sulphureus ?
-
 ?
3.2.1.8 xylopentaose + H2O
-
 Laetiporus sulphureus xylobiose + xylotriose + xylotetraose
-
 ?
3.2.1.8 xylotetraose + H2O
-
 Laetiporus sulphureus 2 xylobiose
-
 ?
3.2.1.8 xylotriose + H2O
-
 Laetiporus sulphureus xylobiose + D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 monomer 1 * 70000, SDS-PAGE, 1 * 69300, LabChip microfluidic system Laetiporus sulphureus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endoxylanase
-
 Laetiporus sulphureus
3.2.1.8 xylanase
-
 Laetiporus sulphureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 80
-
-
 Laetiporus sulphureus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 80 90 activity decreases rapidly at temperatures over 80°C, but still retains approximately 70% relative activity at 90°C Laetiporus sulphureus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 60
-
 retains approximately 97% of its relative activity following incubation for 4 h Laetiporus sulphureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 3
-
-
 Laetiporus sulphureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 2.5 5 maintains relative activity over 50% Laetiporus sulphureus