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Literature summary extracted from

  • Tamura, A.; Yoshikane, Y.; Yokochi, N.; Yagi, T.
    Synthesis of 4-pyridoxolactone from pyridoxine using a combination of transformed Escherichia coli cells (2008), J. Biosci. Bioeng., 106, 460-465.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.12 synthesis development of a simple and efficient synthesis for 4-pyridoxolactone starting with pyridoxine and using a whole-cell biotransformation by two transformed Escherichia coli cell type expressing the pyridoxine oxidase and the catalase, and chaperonin, while the second set expressed pyridoxal 4-dehydrogenase, overview. Pyridoxine is first oxidized to pyridoxal, which is then dehydrogenated to 4-pyridoxolactone by pyridoxine 4-oxidase and pyridoxal 4-dehydrogenase, respectively Microbacterium luteolum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.107 expressed from pET21a vector in Escherichia coli BL21(DE3) Escherichia coli
1.1.3.12 overexpression in Escherichia coli strains JM109 and BL21(DE3), co-expression with catalase, chaperonin, and pyridoxal 4-dehydrogenase for construction of a biotransformation system catalyzing the formation of 4-pyridoxolactone from pyridoxine in whole cells, overview Microbacterium luteolum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.12 pyridoxine + O2 Microbacterium luteolum
-
 pyridoxal + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.107 Escherichia coli
-
-
-
1.1.1.107 Escherichia coli JM109
-
-
-
1.1.3.12 Microbacterium luteolum Q9AJD6
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.107 13.7
-
 Escherichia coli BL21(DE3) cells transformed with pET21a-adh-pld Escherichia coli
1.1.1.107 47.6
-
 Escherichia coli BL21(DE3) cells transformed with pET21a-pld Escherichia coli
1.1.3.12 0.4
-
 recombinant enzyme in Escherichia coli cells Microbacterium luteolum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.107 pyridoxal + NAD+ cells coexpressing pyridoxal 4-dehydrogenase and alcohol dehydrogenase show a higher conversion rate than those expressing pyridoxal 4-dehydrogenase alone Escherichia coli 4-pyridoxolactone + NADH + H+
-
 ?
1.1.1.107 pyridoxal + NAD+ cells coexpressing pyridoxal 4-dehydrogenase and alcohol dehydrogenase show a higher conversion rate than those expressing pyridoxal 4-dehydrogenase alone Escherichia coli JM109 4-pyridoxolactone + NADH + H+
-
 ?
1.1.3.12 pyridoxine + O2
-
 Microbacterium luteolum pyridoxal + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.107 PLDH
-
 Escherichia coli
1.1.3.12 PNOX
-
 Microbacterium luteolum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.12 30
-
 assay at Microbacterium luteolum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.12 7.5
-
 assay at Microbacterium luteolum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.107 NAD+
-
 Escherichia coli