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Literature summary extracted from

  • Shimamura, M.; Nishiyama, T.; Shinya, K.; Kawahara, Y.; Furukawa, K.; Fujii, T.
    Another multiheme protein, hydroxylamine oxidoreductase, abundantly produced in an anammox bacterium besides the hydrazine-oxidizing enzyme (2008), J. Biosci. Bioeng., 105, 243-248.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.8 hao gene, indicating DNA and amino acid sequence determination and analysis Candidatus Jettenia caeni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.8 0.025
-
 hydrazine pH 8.0, 35°C Candidatus Jettenia caeni
1.7.2.8 0.033
-
 hydroxylamine pH 8.0, 35°C Candidatus Jettenia caeni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.8 53000
-
 2 * 53000, SDS-PAGE Candidatus Jettenia caeni
1.7.2.8 118000
-
 gel filtration Candidatus Jettenia caeni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.8 additional information Candidatus Jettenia caeni HAO is an indispensable enzyme and well conserved in anammox bacteria ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.8 Candidatus Jettenia caeni
-
-
-
1.7.2.8 Candidatus Jettenia caeni A9ZRZ6 gene hao
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.8 hydroxylamine oxidoreductase (HAO) is purified from anammox sludge in which an anammox bacterium, strain KSU-1, is dominant Candidatus Jettenia caeni
1.7.2.8 native enzyme 15fold by anion exchange chromatography and gel filtration Candidatus Jettenia caeni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.8 9.6
-
-
 Candidatus Jettenia caeni
1.7.2.8 9.6
-
 partially purified native enzyme Candidatus Jettenia caeni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.8 hydrazine + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide reduction of 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide via phenazine methosulfate Candidatus Jettenia caeni ? + reduced methylthiazolyltetrazolium bromide
-
 ?
1.7.2.8 hydrazine + NH3 + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
-
 Candidatus Jettenia caeni ?
-
 ?
1.7.2.8 hydrazine + NH3 + phenazine methosulfate
-
 Candidatus Jettenia caeni ?
-
 ?
1.7.2.8 hydroxylamine + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide reduction of methylthiazolyltetrazolium bromide via phenazine methosulfate Candidatus Jettenia caeni ? + reduced 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
-
 ?
1.7.2.8 hydroxylamine + NH3 + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
-
 Candidatus Jettenia caeni ?
-
 ?
1.7.2.8 hydroxylamine + NH3 + phenazine methosulfate
-
 Candidatus Jettenia caeni NO2 + H2O + reduced phenazine methosulfate
-
 ?
1.7.2.8 additional information HAO is an indispensable enzyme and well conserved in anammox bacteria Candidatus Jettenia caeni ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.8 dimer 2 * 53000, SDS-PAGE Candidatus Jettenia caeni

Synonyms

EC Number Synonyms Comment Organism
1.7.2.8 HAO
-
 Candidatus Jettenia caeni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.2.8 35
-
 assay at Candidatus Jettenia caeni
1.7.2.8 65
-
-
 Candidatus Jettenia caeni

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.2.8 60
-
 pH 8.0, 2 min, stable below Candidatus Jettenia caeni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.8 8
-
 assay at Candidatus Jettenia caeni
1.7.2.8 8 8.5
-
 Candidatus Jettenia caeni

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.2.8 5 9 35°C, 20 min, stable Candidatus Jettenia caeni

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.8 cytochrome P468 chromophore Candidatus Jettenia caeni
1.7.2.8 cytochrome P468 the enzyme has a cytochrome P468 chromophore Candidatus Jettenia caeni
1.7.2.8 heme c-heme content is 16.1 per 18000 Da protein Candidatus Jettenia caeni
1.7.2.8 heme the hao gene indicats eight c-type heme binding motifs and is a multiheme protein containing covalently bound c-type heme Candidatus Jettenia caeni
1.7.2.8 additional information the sequence deduced from the hao gene indicates eight c-type heme binding motifs Candidatus Jettenia caeni
1.7.2.8 phenazine methosulfate
-
 Candidatus Jettenia caeni