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Literature summary extracted from

  • Tayefi-Nasrabadi, H.
    Some biochemical properties of catalase from kohlrabi (Brassica oleracea gongylodes) (2008), J. Biol. Sci., 8, 649-653.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 azide catalase activity at pH 4.5, in comparison to the other two pH optima at 6.5 and 10.0, has highest sensitivity to azide Brassica oleracea
1.11.1.6 cyanide catalase activity at pH 4.5, in comparison to the other two pH optima at 6.5 and 10.0, has lowest sensitivity to cyanide Brassica oleracea
1.11.1.6 H2O2 catalase activity at pH 4.5 is inhibited by 5 mM H2O2 and above Brassica oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.6 0.64
-
 H2O2 isoenzyme with optimum at pH 6.5 Brassica oleracea
1.11.1.6 1
-
 H2O2 isoenzyme with optimum at pH 4.5 Brassica oleracea
1.11.1.6 13.4
-
 H2O2 isoenzyme with optimum at pH 10.0 Brassica oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 258000
-
 isoenzyme with optimum at pH 10.0, SDS-PAGE Brassica oleracea
1.11.1.6 285000
-
 isoenzyme with optimum at pH 6.5, SDS-PAGE Brassica oleracea
1.11.1.6 308000
-
 isoenzyme with optimum at pH 4.5, SDS-PAGE Brassica oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Brassica oleracea
-
 Brassica oleracea gongylodes, kohlrabi
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.6 bulb
-
 Brassica oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2
-
 Brassica oleracea O2 + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.6 25 70 when the extract is incubated at specified temperature (25-70°C) for various length of time (5-30 min) then tested at room temperature a decrease in catalase activity is found at temperature above 50, 60, and 70°C for pHs of 6.5, 10.0, and 4.5, respectively. After heating at 70°C for 30 min, catalase activity loses about 40, 60, and 75% of the original activity at pH 4.5, 10.0, and 6.5, respectively. Brassica oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 4.5
-
 there are three isoenzymes with pH-optima at 4.5, 6.5, and 10.0 Brassica oleracea
1.11.1.6 6.5
-
 there are three isoenzymes with pH-optima at 4.5, 6.5, and 10.0 Brassica oleracea
1.11.1.6 10
-
 there are three isoenzymes with pH-optima at 4.5, 6.5, and 10.0 Brassica oleracea

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.11.1.6 0.01
-
 isoenzyme with optimum at pH 4.5 Brassica oleracea azide
1.11.1.6 0.075
-
 isoenzyme with optimum at pH 6.5 Brassica oleracea azide
1.11.1.6 0.2
-
 isoenzyme with optimum at pH 6.5 Brassica oleracea cyanide
1.11.1.6 1
-
 isoenzyme with optimum at pH 10.0 Brassica oleracea azide
1.11.1.6 2
-
 isoenzyme with optimum at pH 10.0 Brassica oleracea cyanide
1.11.1.6 12
-
 isoenzyme with optimum at pH 4.5 Brassica oleracea cyanide