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Literature summary extracted from

  • Petruccioli, M.; Frasconi, M.; Quaratino, D.; Covino, S.; Favero, G.; Mazzei, F.; Federici, F.; DAnnibale, A.
    Kinetic and redox properties of MnP II, a major manganese peroxidase isoenzyme from Panus tigrinus CBS 577.79 (2009), J. Biol. Inorg. Chem., 14, 1153-1163.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.13 diphosphate diphosphate proves to be the worst chelator, leading to a specific activity about 4.6% of that obtained with tartrate Lentinus tigrinus
1.11.1.13 Lactate lactate serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 48.4% of that obtained with tartrate Lentinus tigrinus
1.11.1.13 malonate the highest stimulation of 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate, malonate also serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 54.8% of that obtained with tartrate Lentinus tigrinus
1.11.1.13 oxalate oxalate serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 64.7% of that obtained with tartrate Lentinus tigrinus
1.11.1.13 Tartrate the highest rate of Mn3+ formation is obtained with 10 mM tartrate Lentinus tigrinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.13 0.016
-
 H2O2
-
 Lentinus tigrinus
1.11.1.13 0.016
-
 H2O2 pH 5.5, 45°C Lentinus tigrinus
1.11.1.13 0.0713
-
 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid)
-
 Lentinus tigrinus
1.11.1.13 0.0713
-
 2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus
1.11.1.13 0.076
-
 2,6-dimethoxyphenol
-
 Lentinus tigrinus
1.11.1.13 0.076
-
 2,6-dimethoxyphenol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
1.11.1.13 0.124
-
 Mn2+
-
 Lentinus tigrinus
1.11.1.13 0.124
-
 Mn2+ pH 5.5, 45°C Lentinus tigrinus
1.11.1.13 0.196
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
 Lentinus tigrinus
1.11.1.13 0.196
-
 2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
1.11.1.13 0.213
-
 guaiacol
-
 Lentinus tigrinus
1.11.1.13 0.213
-
 guaiacol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
1.11.1.13 0.293
-
 pyrogallol
-
 Lentinus tigrinus
1.11.1.13 0.293
-
 pyrogallol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
1.11.1.13 0.56
-
 veratryl alcohol
-
 Lentinus tigrinus
1.11.1.13 0.56
-
 veratryl alcohol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
1.11.1.13 2.3
-
 guaiacol
-
 Lentinus tigrinus
1.11.1.13 2.3
-
 guaiacol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus
1.11.1.13 3.74
-
 pyrogallol
-
 Lentinus tigrinus
1.11.1.13 3.74
-
 pyrogallol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus
1.11.1.13 6.954
-
 2,6-dimethoxyphenol
-
 Lentinus tigrinus
1.11.1.13 6.954
-
 2,6-dimethoxyphenol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.13 Manganese
-
 Lentinus tigrinus
1.11.1.13 Mn2+ veratryl alcohol oxidation requires the simultaneous presence of H2O2 and Mn2+ Lentinus tigrinus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.13 50500
-
 gel filtration Lentinus tigrinus
1.11.1.13 50500
-
 determined by gel filtration and SDS-PAGE Lentinus tigrinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.13 Mn2+ + H+ + H2O2 Lentinus tigrinus
-
 Mn3+ + H2O
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2 Lentinus tigrinus CBS 577.79
-
 Mn3+ + H2O
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2 Lentinus tigrinus 577.79
-
 Mn3+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.13 Lentinus tigrinus
-
-
-
1.11.1.13 Lentinus tigrinus 577.79
-
-
-
1.11.1.13 Lentinus tigrinus CBS 577.79
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.13 glycoprotein N-glycosylation of about 5.3% of the high-mannose type Lentinus tigrinus
1.11.1.13 glycoprotein the enzyme displays an extent of N-glycosylation of about 5.3% of the high-mannose type Lentinus tigrinus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.13 purification scheme involves ultrafiltration, affinity chromatography on concanavalin-A Sepharose and gel filtration Lentinus tigrinus
1.11.1.13 ultrafiltration, Concanavalin-A Sepharose column chromatography and Superdex 75 gel filtration Lentinus tigrinus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.13 40
-
 purification step culture supernatant Lentinus tigrinus
1.11.1.13 40
-
 culture supernatant, pH 5.5, 45°C Lentinus tigrinus
1.11.1.13 41
-
 purification step ultrafiltration Lentinus tigrinus
1.11.1.13 169
-
 purification step chromatography on a concanavalin-A Sepharose column Lentinus tigrinus
1.11.1.13 288
-
 purification step gel filtration Lentinus tigrinus
1.11.1.13 288
-
 after 7.2fold purification, pH 5.5, 45°C Lentinus tigrinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.13 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 + H+
-
 Lentinus tigrinus ?
-
 r
1.11.1.13 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ?
-
 Lentinus tigrinus ?
-
 ?
1.11.1.13 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ?
-
 Lentinus tigrinus CBS 577.79 ?
-
 ?
1.11.1.13 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ?
-
 Lentinus tigrinus 577.79 ?
-
 ?
1.11.1.13 2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid + H2O2
-
 Lentinus tigrinus ?
-
 ?
1.11.1.13 2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid + H2O2 + Mn2+
-
 Lentinus tigrinus ?
-
 ?
1.11.1.13 2,6-dimethoxyphenol + H2O2 the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate Lentinus tigrinus ?
-
 ?
1.11.1.13 2,6-dimethoxyphenol + H2O2 the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate Lentinus tigrinus CBS 577.79 ?
-
 ?
1.11.1.13 2,6-dimethoxyphenol + H2O2 the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate Lentinus tigrinus 577.79 ?
-
 ?
1.11.1.13 2,6-dimethoxyphenol + H2O2 + Mn2+
-
 Lentinus tigrinus ?
-
 ?
1.11.1.13 2,6-dimethoxyphenol + Mn2+ + ?
-
 Lentinus tigrinus ?
-
 ?
1.11.1.13 guaiacol + H2O2
-
 Lentinus tigrinus ?
-
 ?
1.11.1.13 guaiacol + H2O2
-
 Lentinus tigrinus CBS 577.79 ?
-
 ?
1.11.1.13 guaiacol + H2O2
-
 Lentinus tigrinus 577.79 ?
-
 ?
1.11.1.13 guaiacol + H2O2 + Mn2+
-
 Lentinus tigrinus ?
-
 ?
1.11.1.13 guaiacol + Mn2+ + ?
-
 Lentinus tigrinus ?
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Lentinus tigrinus Mn3+ + H2O
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Lentinus tigrinus CBS 577.79 Mn3+ + H2O
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Lentinus tigrinus 577.79 Mn3+ + H2O
-
 ?
1.11.1.13 pyrogallol + H2O2
-
 Lentinus tigrinus ?
-
 ?
1.11.1.13 pyrogallol + H2O2
-
 Lentinus tigrinus CBS 577.79 ?
-
 ?
1.11.1.13 pyrogallol + H2O2
-
 Lentinus tigrinus 577.79 ?
-
 ?
1.11.1.13 pyrogallol + H2O2 + Mn2+
-
 Lentinus tigrinus ?
-
 ?
1.11.1.13 pyrogallol + Mn2+ + ?
-
 Lentinus tigrinus ?
-
 ?
1.11.1.13 veratryl alcohol + H2O2 + Mn2+ veratryl alcohol oxidation requires the simultaneous presence of H2O2 and Mn2+ Lentinus tigrinus ?
-
 ?
1.11.1.13 veratryl alcohol + Mn2+ + ?
-
 Lentinus tigrinus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.13 monomer
-
 Lentinus tigrinus
1.11.1.13 monomer 1 * 50500, SDS-PAGE Lentinus tigrinus

Synonyms

EC Number Synonyms Comment Organism
1.11.1.13 manganese peroxidase
-
 Lentinus tigrinus
1.11.1.13 Mn2+: hydrogen peroxide oxidoreductase
-
 Lentinus tigrinus
1.11.1.13 Mn2+:hydrogen peroxide oxidoreductase
-
 Lentinus tigrinus
1.11.1.13 MnP II
-
 Lentinus tigrinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.13 45
-
-
 Lentinus tigrinus
1.11.1.13 45
-
 optimum temperature for the oxidation of Mn2+ and DMOP Lentinus tigrinus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.11.1.13 10 60 the enzyme shows relative activities of 34, 50, and 47% at 10, 40, and 60°C, respectively Lentinus tigrinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.13 50 70 the enzyme is rather unstable at 50, 60, and 70°C, resulting in half-lives of 660, 105, and 41 s, respectively Lentinus tigrinus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.13 0.45
-
 veratryl alcohol
-
 Lentinus tigrinus
1.11.1.13 0.45
-
 veratryl alcohol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
1.11.1.13 0.74
-
 guaiacol
-
 Lentinus tigrinus
1.11.1.13 0.74
-
 guaiacol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus
1.11.1.13 1.87
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) Mn2+
-
 Lentinus tigrinus
1.11.1.13 1.87
-
 2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus
1.11.1.13 5.67
-
 2,6-dimethoxyphenol
-
 Lentinus tigrinus
1.11.1.13 5.67
-
 2,6-dimethoxyphenol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus
1.11.1.13 8.92
-
 guaiacol
-
 Lentinus tigrinus
1.11.1.13 8.92
-
 guaiacol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
1.11.1.13 9.98
-
 pyrogallol
-
 Lentinus tigrinus
1.11.1.13 9.98
-
 pyrogallol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C Lentinus tigrinus
1.11.1.13 20.2
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
 Lentinus tigrinus
1.11.1.13 20.2
-
 2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
1.11.1.13 20.95
-
 pyrogallol
-
 Lentinus tigrinus
1.11.1.13 20.95
-
 pyrogallol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
1.11.1.13 26.26
-
 2,6-dimethoxyphenol
-
 Lentinus tigrinus
1.11.1.13 26.26
-
 2,6-dimethoxyphenol in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C Lentinus tigrinus
1.11.1.13 121
-
 H2O2
-
 Lentinus tigrinus
1.11.1.13 121
-
 H2O2 with 1.5 mM Mn2+ as cosubstrate, pH 5.5, 45°C Lentinus tigrinus
1.11.1.13 242.4
-
 Mn2+
-
 Lentinus tigrinus
1.11.1.13 242.4
-
 Mn2+ with 0.2 mM H2O2 as cosubstrate, pH 5.5, 45°C Lentinus tigrinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.13 3
-
 optimal pH for the Mn-dependent oxidation of veratryl alcohol Lentinus tigrinus
1.11.1.13 3
-
 optimum pH for the Mn2+-dependent oxidation of veratryl alehyde Lentinus tigrinus
1.11.1.13 3.5 4.5 pH optimum for Mn2+-independent DMOP oxidation Lentinus tigrinus
1.11.1.13 4 4.5 pH optimum for Mn2+-dependent DMOP oxidation Lentinus tigrinus
1.11.1.13 5.5
-
-
 Lentinus tigrinus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.13 3.5 6.5 Mn2+ oxidation shows an optimum in the range 5.5-5.75 and negligible activity at pH values below 3.5 and above 6.5 Lentinus tigrinus
1.11.1.13 3.5 4.5 optima for Mn2+-independent 2,6-dimethoxyphenol oxidation Lentinus tigrinus
1.11.1.13 4 4.5 optima for Mn2+-dependent 2,6-dimethoxyphenol oxidation Lentinus tigrinus
1.11.1.13 5.5 5.8 the pH-activity profile for Mn2+ oxidation shows an optimum in the range 5.5 - 5.75 and negligible activity at pHs below 3.5 and above 6.5 Lentinus tigrinus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.13 3.5 5.5 the enzyme shows a half-life of 8.8 h at pH 3.5, the half-lives of MnP activity at pH 4.5, 5.0, and 5.5 are 89.5, 99, and 101 h, respectively Lentinus tigrinus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.13 heme the heme content is 0.96 mol per mole of the enzyme Lentinus tigrinus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.13 Lentinus tigrinus
-
-
 4.07
1.11.1.13 Lentinus tigrinus isoelectric focusing
-
 4.1