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Literature summary extracted from

  • Pamuklar, Z.; Federico, L.; Liu, S.; Umezu-Goto, M.; Dong, A.; Panchatcharam, M.; Fulerson, Z.; Berdyshev, E.; Natarajan, V.; Fang, X.; van Meeteren, L.A.; Moolenaar, W.H.; Mills, G.B.; Morris, A.J.; Smyth, S.S.
    Autotaxin/lysopholipase D and lysophosphatidic acid regulate murine hemostasis and thrombosis (2009), J. Biol. Chem., 284, 7385-7394.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.39 generate mouse overexpressing circulating autotaxin: amplification of human 1-antitrypsin (A1AT) promoter by PCR (from a Yac clone obtained from ATCC), injection of DNA into pronuclei of 1-cellstage FVB mouse embryos, transfer of injected cells into the oviduct of pseudopregnant female mouse. Transfection of CHO cells with a vector for expression of a fusion protein containing the human immunoglobulin-kappa chain leader sequence, a His-tag, and a cDNA-sequence encoding aminoacids 52-915 of human autotaxin-gamma Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.39 lysophosphatic acid
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.39 100000
-
 immunoblotting, 3D1 antibody (1/500), a polyclonal antibody raised against the peptide sequence in autotaxin CKKPDQHFKPYMKQHLPKRL and a polyclonal antibody to amino acids 541-720 of autotoxin Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.39 lysophosphatidylcholine + H2O Homo sapiens
-
 lysophosphatidic acid + choline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.39 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.4.39 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.39 from CHO cells: sequential metal ion affinity and gel filtration chromatography, exchanged into phosphate-buffered saline and concentrated to 1 mg/ml of monomeric autotaxin by centrifugal filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.39 lysophosphatidylcholine + H2O
-
 Homo sapiens lysophosphatidic acid + choline
-
 ?
3.1.4.39 lysophosphatidylcholine + H2O radiolabeled substrate and fluorogenic substrate FS-3 Homo sapiens lysophosphatidic acid + choline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.39 autotaxin
-
 Homo sapiens
3.1.4.39 ENPP2
-
 Homo sapiens
3.1.4.39 lysoPLD
-
 Homo sapiens