Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Johnsen, U.; Dambeck, M.; Zaiss, H.; Fuhrer, T.; Soppa, J.; Sauer, U.; Schoenheit, P.
    D-xylose degradation pathway in the halophilic archaeon Haloferax volcanii (2009), J. Biol. Chem., 284, 27290-27303.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.179 additional information induced by D-xylose Haloferax volcanii
4.2.1.82 xylose xylonate dehydratase activity is induced 2fold in xylose-grown cells Haloferax volcanii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.179 cloned into pET19b, resulting plasmid harvested from Escherichia coli JM109, sequenced, and transformed into Escherichia coli Rosetta(DE3)-pLysS expression strain Haloferax volcanii
1.1.1.179 expression in Escherichia coli Haloferax volcanii
4.2.1.82 PCR product cloned into pET19b. The resulting plasmid harvested from Escherichia coli JM109, sequenced, and transformed into Escherichia coli Rosetta(DE3)-pLysS expression strain. In-frame deletion mutant amplified and ligated into pTA131, sequenced, and transformed in Haloferax volcanii H26 DELTApyrE2 Haloferax volcanii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.179 additional information in-frame deletion mutant can not grow on xylose but grows unaffected on glucose as the wild-type Haloferax volcanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.179 0.031
-
 NADP+
-
 Haloferax volcanii
1.1.1.179 0.36
-
 NAD+
-
 Haloferax volcanii
1.1.1.179 0.75
-
 NADP+ pH 8.3, 42°C Haloferax volcanii
1.1.1.179 4.7
-
 D-xylose
-
 Haloferax volcanii
1.1.1.179 8 9 D-xylose pH 8.3, 42°C Haloferax volcanii
1.1.1.179 198
-
 D-glucose
-
 Haloferax volcanii
4.2.1.82 0.4
-
 D-gluconate at 42°C, in 0.1 M Tris-HCl buffer, pH 7.5, 2.5 M KCl, 50 mM MgCl2 Haloferax volcanii
4.2.1.82 0.55
-
 D-xylonate at 42°C, in 0.1 M Tris-HCl buffer, pH 7.5, 2.5 M KCl, 50 mM MgCl2 Haloferax volcanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.82 Mg2+
-
 Haloferax volcanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.179 5550
-
 4 * 5550, SDS-PAGE. 4 * 42300, sequence analysis Haloferax volcanii
1.1.1.179 42300
-
 4 * 5550, SDS-PAGE. 4 * 42300, sequence analysis Haloferax volcanii
1.1.1.179 165000
-
 native enzyme Haloferax volcanii
4.2.1.82 45500
-
 8 * 45500, sequence analysis. 8 * 52000, gel filtration Haloferax volcanii
4.2.1.82 52000
-
 8 * 45500, sequence analysis. 8 * 52000, gel filtration Haloferax volcanii
4.2.1.82 340000
-
 gel filtration Haloferax volcanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.179 D-xylose + NADP+ Haloferax volcanii initial step in xylose degradation D-xylono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.179 D-xylose + NADP+ Haloferax volcanii DS2 initial step in xylose degradation D-xylono-1,5-lactone + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.179 Haloferax volcanii D4GP29 DS70 strain H26
-
1.1.1.179 Haloferax volcanii D4GP30
-
-
1.1.1.179 Haloferax volcanii DS2 D4GP30
-
-
4.2.1.82 Haloferax volcanii D4GP40
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.179 on Ni-NTA column and by gel filtration Haloferax volcanii
4.2.1.82 by centrifugation, gel filtration, ammonium sulfate precipitation and ultrafiltration, 100fold. Recombinant XAD purified by Ni-NTA chromatography Haloferax volcanii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.179 culture condition:xylose-grown cell
-
 Haloferax volcanii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.179 7.8
-
 with D-glucose as substrate Haloferax volcanii
1.1.1.179 10
-
 pH 8.3, 42°C Haloferax volcanii
1.1.1.179 24.1
-
 with D-xylose as substrate Haloferax volcanii
4.2.1.82 1.1
-
 100fold purified enzyme, with D-xylonate or D-gluconate as substrate Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.179 D-glucose + NAD(P)+
-
 Haloferax volcanii D-gluconolactone + NAD(P)H
-
 ?
1.1.1.179 D-glucose + NADP+ 20% of the activity with D-xylose Haloferax volcanii D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.179 D-glucose + NADP+ 20% of the activity with D-xylose Haloferax volcanii DS2 D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.179 D-xylose + NAD(P)+ is highly specific for D-xylose Haloferax volcanii D-xylonolactone + NAD(P)H
-
 ?
1.1.1.179 D-xylose + NAD+
-
 Haloferax volcanii D-xylono-1,5-lactone + NADH + H+
-
 r
1.1.1.179 D-xylose + NADP+
-
 Haloferax volcanii D-xylono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.179 D-xylose + NADP+ initial step in xylose degradation Haloferax volcanii D-xylono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.179 D-xylose + NADP+
-
 Haloferax volcanii DS2 D-xylono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.179 D-xylose + NADP+ initial step in xylose degradation Haloferax volcanii DS2 D-xylono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.179 additional information no significant oxidation with L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose Haloferax volcanii ?
-
 ?
4.2.1.82 D-gluconate XAD catalyzes the dehydration of D-gluconate at a similar catalytic efficiency as compared with D-xylonate Haloferax volcanii 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
4.2.1.82 D-xylonate XAD catalyzes the dehydration of D-xylonate at a similar catalytic efficiency as compared with D-gluconate Haloferax volcanii 2-dehydro-3-deoxy-D-arabinonate + H2O
-
 ?
4.2.1.82 additional information D-galactonate is not utilized Haloferax volcanii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.179 tetramer 4 * 5550, SDS-PAGE. 4 * 42300, sequence analysis Haloferax volcanii
4.2.1.82 homooctamer 8 * 45500, sequence analysis. 8 * 52000, gel filtration Haloferax volcanii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.179 D-xylose dehydrogenase
-
 Haloferax volcanii
1.1.1.179 XDH
-
 Haloferax volcanii
4.2.1.82 D-xylonate dehydratase
-
 Haloferax volcanii
4.2.1.82 XAD
-
 Haloferax volcanii
4.2.1.82 xylonate dehydratase
-
 Haloferax volcanii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.179 NAD(P)+
-
 Haloferax volcanii
1.1.1.179 NADP+ no activity with NAD+ Haloferax volcanii

General Information

EC Number General Information Comment Organism
4.2.1.82 malfunction in-frame deletion mutant has lost its ability to grow on xylose, but it shows the same phenotype as the wild-type during growth on glucose Haloferax volcanii