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Literature summary extracted from

  • Gesell, A.; Rolf, M.; Ziegler, J.; Diaz Chavez, M.L.; Huang, F.C.; Kutchan, T.M.
    CYP719B1 is salutaridine synthase, the C-C phenol-coupling enzyme of morphine biosynthesis in opium poppy (2009), J. Biol. Chem., 284, 24432-24442.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.67 full-length CYP719B1 cDNA is generated by amplification out of bacterial vector pCR2.1 and inserted into baculovirus transfer vector pVL1392 Papaver somniferum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.67 0.0062
-
 (R)-reticuline
-
 Papaver somniferum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.67 50000
-
 determined by SDS-PAGE Papaver somniferum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.67 (R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2 Papaver somniferum
-
 salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.67 Papaver somniferum B1NF18
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.67 microsomes from insect cells are prepared Papaver somniferum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.67 (R)-norreticuline + [reduced NADPH-hemoprotein reductase] + O2
-
 Papaver somniferum norsalutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.19.67 (R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
-
 Papaver somniferum salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.67 CYP719B1
-
 Papaver somniferum
1.14.19.67 salutaridine synthase
-
 Papaver somniferum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.67 30
-
 assay for kinetic analysis Papaver somniferum
1.14.19.67 37
-
 activity assay Papaver somniferum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.19.67 0.027
-
 (R)-reticuline
-
 Papaver somniferum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.19.67 6.5
-
 activity assay Papaver somniferum
1.14.19.67 8
-
 assay for kinetic analysis Papaver somniferum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.67 cytochrome P450
-
 Papaver somniferum