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Literature summary extracted from

  • Yalcin, A.; Clem, B.F.; Simmons, A.; Lane, A.; Nelson, K.; Clem, A.L.; Brock, E.; Siow, D.; Wattenberg, B.; Telang, S.; Chesney, J.
    Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases (2009), J. Biol. Chem., 284, 24223-24232.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.105 both parent and double mutant (K472A/K473A) constructs are transformed into the BL21 strain of Escherichia coli, overexpression of PFKFB3 causes no change in glucose metabolism but rather a marked increase in cell proliferation Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.105 K472A/K473A cytoplasmic mutant Homo sapiens
2.7.1.105 R75A/R76A kinase inactive Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.105 cytoplasm
-
 Homo sapiens 5737
-
2.7.1.105 nucleus PFKFB3 splice variant 5, the carboxyl terminus is required for this localization Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate Homo sapiens
-
 ADP + beta-D-fructose 2,6-bisphosphate regulation of glycolysis ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Homo sapiens Q16875
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 HCT-116 cell
-
 Homo sapiens
-
2.7.1.105 HeLa cell
-
 Homo sapiens
-
2.7.1.105 MDA-MB-231 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.105 additional information
-
 nuclei isolated from cells transfected with wild type PFKFB3 exhibit 6-phosphofructo-2-kinase activity that is significantly higher than the nuclei obtained from both vector and K472A/K473A-transfected cells Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Homo sapiens ADP + beta-D-fructose 2,6-bisphosphate regulation of glycolysis ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.105 6-phosphofructo-2-kinase
-
 Homo sapiens
2.7.1.105 PFKFB3
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.105 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.105 7.5
-
 assay at Homo sapiens

Expression

EC Number Organism Comment Expression
2.7.1.105 Homo sapiens ectopic expression of wild type PFKFB3 increases phosphorylation of the cell cycle inhibitor p27, a member of the Kip/Cip family of proteins and a universal inhibitor of cyclin-dependent kinases and the cell cycle, at threonine 187 and decreases total p27 protein levels additional information

General Information

EC Number General Information Comment Organism
2.7.1.105 malfunction PFKFB3 enzymes are activated in human cancers Homo sapiens
2.7.1.105 physiological function PFKFB3 has a role in nuclear signaling Homo sapiens