BRENDA - Enzyme Database

Deglutathionylation of 2-Cys peroxiredoxin is specifically catalyzed by sulfiredoxin

Park, J.W.; Mieyal, J.J.; Rhee, S.G.; Chock, P.B.; J. Biol. Chem. 284, 23364-23374 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.8.98.2
for expression in Escherichia coli BL21DE3 cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.8.98.2
H100N
Srx mutant
Homo sapiens
1.8.98.2
R101M
Srx mutant
Homo sapiens
1.8.98.2
Y92R
Srx mutant
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.98.2
siRNA
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.98.2
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH
Homo sapiens
-
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.98.2
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.98.2
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.98.2
A-549 cell
-
Homo sapiens
-
1.8.98.2
HeLa cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.2
additional information
catalyzes the deglutathionylation of actin
698928
Homo sapiens
?
-
-
-
-
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH
-
698928
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.98.2
ATP
-
Homo sapiens
1.8.98.2
glutathione
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.98.2
for expression in Escherichia coli BL21DE3 cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.98.2
ATP
-
Homo sapiens
1.8.98.2
glutathione
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.8.98.2
H100N
Srx mutant
Homo sapiens
1.8.98.2
R101M
Srx mutant
Homo sapiens
1.8.98.2
Y92R
Srx mutant
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.98.2
siRNA
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.98.2
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH
Homo sapiens
-
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.98.2
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.98.2
A-549 cell
-
Homo sapiens
-
1.8.98.2
HeLa cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.2
additional information
catalyzes the deglutathionylation of actin
698928
Homo sapiens
?
-
-
-
-
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + R-SH
-
698928
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?