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Literature summary extracted from

  • Winkler, A.; Motz, K.; Riedl, S.; Puhl, M.; Macheroux, P.; Gruber, K.
    Structural and mechanistic studies reveal the functional role of bicovalent flavinylation in berberine bridge enzyme (2009), J. Biol. Chem., 284, 19993-20001.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.3.3 for co-overexpression with disulfide isomerase in Pichia pastoris cells, for cloning of wild-type BBE, the mutant H104A and mutant H104A-C166A the vector pPICZalpha is used Eschscholzia californica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.21.3.3 the crystal structures of the mutants H104A, C166A, C166A in complex with (S)-reticuline and of the wild-type enzyme in complex with (S)-scoulerine are determined Eschscholzia californica

Protein Variants

EC Number Protein Variants Comment Organism
1.21.3.3 C166A mutant, lacking one of the covalent linkages to the cofactor FAD Eschscholzia californica
1.21.3.3 H104A mutant, lacking one of the covalent linkages to the cofactor FAD Eschscholzia californica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.3 (S)-reticuline + O2 Eschscholzia californica
-
 (S)-scoulerine + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.3 Eschscholzia californica P30986
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.3.3 the secreted enzymes are purified by gel filtration and anion exchange chromatography Eschscholzia californica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.3 (S)-reticuline + O2
-
 Eschscholzia californica (S)-scoulerine + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.21.3.3 BBE
-
 Eschscholzia californica
1.21.3.3 berberine bridge enzyme
-
 Eschscholzia californica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.21.3.3 0.48
-
 (S)-reticuline mutant C166A Eschscholzia californica
1.21.3.3 0.54
-
 (S)-reticuline mutant H104A Eschscholzia californica
1.21.3.3 8
-
 (S)-reticuline wild-type Eschscholzia californica

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.3.3 FAD
-
 Eschscholzia californica