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Literature summary extracted from

  • Horie, Y.; Ito, H.; Kusaba, M.; Tanaka, R.; Tanaka, A.
    Participation of chlorophyll b reductase in the initial step of the degradation of light-harvesting chlorophyll a/b-protein complexes in Arabidopsis (2009), J. Biol. Chem., 284, 17449-17456.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.294 coding region of chlorophyll b reductase (NOL) lacking its transit peptide amplified and cloned into pET-30a(+) at NspV and HindIII sites, expressed in Escherichia coli Rosetta DE3 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.294 additional information Arabidopsis nol/nyc1 double mutant, when the genes for chlorophyll b reductases NOL and NYC1 are disrupted, chlorophyll b and photosystem II are not degraded during senescence, whereas other pigment complexes completely disappear Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.294 additional information chlorophyll b reductase decreases during senescence and finally disappears after 8-day-dark incubation Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.294 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.294 by centrifugation, sonication, on a nickel column and by ultrafiltration Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
1.1.1.294 4°C, 24 hours Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.294 chlorophyll b + NADPH when purified trimeric photosystem II is incubated with recombinant chlorophyll b reductase (NOL), conversion of chlorophyll b in photosystem II to 7-hydroxymethyl chlorophyll a. Chlorophyll b reductase catalyzes the initial step of photosystem II degradation Arabidopsis thaliana 7-hydroxymethyl chlorophyll a + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.294 chlorophyll b reductase
-
 Arabidopsis thaliana
1.1.1.294 NOL
-
 Arabidopsis thaliana
1.1.1.294 NYC1
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.294 NADPH
-
 Arabidopsis thaliana