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Literature summary extracted from

  • Belenky, P.; Christensen, K.C.; Gazzaniga, F.; Pletnev, A.A.; Brenner, C.
    Nicotinamide riboside and nicotinic acid riboside salvage in fungi and mammals. Quantitative basis for Urh1 and purine nucleoside phosphorylase function in NAD+ metabolism (2009), J. Biol. Chem., 284, 158-164.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.3 expressed in Escherichia coli Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.3 immucillin-H
-
 Saccharomyces cerevisiae
3.2.2.3 additional information Urh1 activity is unaffected by EDTA Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.3 0.016
-
 nicotinamide riboside in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae
3.2.2.3 0.045
-
 nicotinic acid riboside methyl ester in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae
3.2.2.3 0.15
-
 nicotinic acid riboside in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae
3.2.2.3 1.6
-
 uridine in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.173 Mg2+ required Saccharomyces cerevisiae
3.2.2.3 additional information Urh1 activity is unaffected by the addition of MgCl2 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.173 ATP + beta-D-ribosylnicotinamide Saccharomyces cerevisiae
-
 ADP + nicotinamide beta-D-ribonucleotide
-
 ?
2.7.1.173 ATP + beta-D-ribosylnicotinamide Saccharomyces cerevisiae BY4742
-
 ADP + nicotinamide beta-D-ribonucleotide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.173 Saccharomyces cerevisiae
-
-
-
3.2.2.3 Saccharomyces cerevisiae
-
-
-
3.2.2.3 Saccharomyces cerevisiae BY4742
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.173 ATP + beta-D-ribosylnicotinamide
-
 Saccharomyces cerevisiae ADP + nicotinamide beta-D-ribonucleotide
-
 ?
2.7.1.173 ATP + beta-D-ribosylnicotinamide
-
 Saccharomyces cerevisiae BY4742 ADP + nicotinamide beta-D-ribonucleotide
-
 ?
3.2.2.3 nicotinamide riboside + H2O the uridine hydrolase is 100fold more active as a nicotinamide riboside hydrolase than as a uridine hydrolase Saccharomyces cerevisiae nicotinamide + D-ribose
-
 ?
3.2.2.3 nicotinamide riboside + H2O the uridine hydrolase is 100fold more active as a nicotinamide riboside hydrolase than as a uridine hydrolase Saccharomyces cerevisiae BY4742 nicotinamide + D-ribose
-
 ?
3.2.2.3 nicotinic acid riboside + H2O
-
 Saccharomyces cerevisiae nicotinic acid + D-ribose
-
 ?
3.2.2.3 nicotinic acid riboside + H2O
-
 Saccharomyces cerevisiae BY4742 nicotinic acid + D-ribose
-
 ?
3.2.2.3 nicotinic acid riboside methyl ester + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.2.2.3 nicotinic acid riboside methyl ester + H2O
-
 Saccharomyces cerevisiae BY4742 ?
-
 ?
3.2.2.3 uridine + H2O
-
 Saccharomyces cerevisiae uracil + D-ribose
-
 ?
3.2.2.3 uridine + H2O
-
 Saccharomyces cerevisiae BY4742 uracil + D-ribose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.173 nicotinamide riboside kinase
-
 Saccharomyces cerevisiae
2.7.1.173 Nrk
-
 Saccharomyces cerevisiae
3.2.2.3 URH1
-
 Saccharomyces cerevisiae
3.2.2.3 uridine-preferring pyrimidine nucleoside hydrolase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.3 1.8
-
 nicotinic acid riboside in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae
3.2.2.3 2.3
-
 nicotinic acid riboside methyl ester in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae
3.2.2.3 20
-
 uridine in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae
3.2.2.3 23.4
-
 nicotinamide riboside in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.173 ATP
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
2.7.1.173 metabolism nicotinamide riboside elevates NAD+ levels via the nicotinamide riboside kinase pathway and by a pathway initiated by splitting the nucleoside into a nicotinamide base followed by nicotinamide salvage. Yeast nicotinic acid riboside utilization largely depends on uridine hydrolase and nicotinamide riboside kinase, and nicotinic acid riboside bioavailability is increased by ester modification Saccharomyces cerevisiae
2.7.1.173 additional information comparison to the Nrk-independent utilization pathway of nicotinamide riboside in yeast, overview Saccharomyces cerevisiae
2.7.1.173 physiological function nicotinamide riboside kinase increases the NAD+ levels via convertion of the substrate nicotinamide riboside and thereby extending replicative lifespan and increases Sir2-dependent gene silencing Saccharomyces cerevisiae