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Literature summary extracted from

  • van Wonderen, J.H.; Burlat, B.; Richardson, D.J.; Cheesman, M.R.; Butt, J.N.
    The nitric oxide reductase activity of cytochrome c nitrite reductase from Escherichia coli (2008), J. Biol. Chem., 283, 9587-9594.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.7.2.2 additional information anaerobically grown Escherichia coli nrf mutants are more sensitive to NO radicals than the parent strain Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.2 CN- cyanide is a potent inhibitor of NrfA nitrite and hydroxylamine reductase activities Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.2 0.012
-
 nitrite pH 7.0, 20°C, reduction Escherichia coli
1.7.2.2 0.3
-
 NO radical pH 7.0, 20°C, reduction Escherichia coli
1.7.2.2 60.4
-
 hydroxylamine pH 7.0, 20°C, reduction Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.2 membrane
-
 Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.2 Fe2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.2 additional information Escherichia coli NrfA from Escherichia coli has a well established role in the respiratory reduction of nitrite to ammonium, it might also participate in NO radical detoxification, detoxifying exogenously generated NO radical encountered during invasion of a human host, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.2 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.2 additional information
-
 quantitation of steady-state NrfA reduction of NO radical, nitrite, and hydroxylamine Escherichia coli
1.7.2.2 25
-
 nitrite reduction Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.2 hydroxylamine + reduced methyl viologen low activity Escherichia coli ?
-
 ?
1.7.2.2 additional information NrfA from Escherichia coli has a well established role in the respiratory reduction of nitrite to ammonium, it might also participate in NO radical detoxification, detoxifying exogenously generated NO radical encountered during invasion of a human host, overview Escherichia coli ?
-
 ?
1.7.2.2 additional information the enzyme shows a common site for reduction of all three substrates as axial ligands to the lysine-coordinated NrfA heme rather than nonspecific NO radical reduction at one of the four His-His coordinated hemes also present in each NrfA subunit. NO radical reduction is initiated at similar potentials to NrfA-catalyzed reduction of nitrite and hydroxylamine Escherichia coli ?
-
 ?
1.7.2.2 nitrite + reduced methyl viologen
-
 Escherichia coli NH3 + H2O + oxidized methyl viologen
-
 ?
1.7.2.2 NO radical + reduced methyl viologen
-
 Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.2 cytochrome c nitrite reductase
-
 Escherichia coli
1.7.2.2 NrfA
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.2.2 20
-
 assay at Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.7.2.2 4 25 NrfA activity increases when the temperature is raised from 4°C to 25°C Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.2 700
-
 nitrite pH 7.0, 20°C, reduction Escherichia coli
1.7.2.2 840
-
 NO radical pH 7.0, 20°C, reduction Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.2 7
-
 assay at Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.2.2 additional information
-
 pH dependence of NrfA at 4-25°C, overview Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.2 heme
-
 Escherichia coli
1.7.2.2 methyl viologen used as the electron donor, reduced with dithionite, artificial cofactor Escherichia coli