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Literature summary extracted from

  • Araie, H.; Suzuki, I.; Shiraiwa, Y.
    Identification and characterization of a selenoprotein, thioredoxin reductase, in a unicellular marine haptophyte alga, Emiliania huxleyi (2008), J. Biol. Chem., 283, 35329-35336.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 52320
-
 isoform SEP1, calculated from amino acid sequence Emiliania huxleyi
1.8.1.9 59000
-
 isoform SEP1, SDS-PAGE Emiliania huxleyi

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Emiliania huxleyi B9A1H3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 DEAE-Toyopearl column chromatography and Diol-150 gel filtration Emiliania huxleyi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.9 14.1
-
 using thioredoxin disulfide and NADPH as substrates, at 25°C Emiliania huxleyi
1.8.1.9 36.4
-
 using thioredoxin disulfide, insulin, and NADPH as substrates, at 25°C Emiliania huxleyi
1.8.1.9 150.6
-
 using 5,5'-dithiobis(2-nitrobenzoic acid) and NADPH as substrates, at 25°C Emiliania huxleyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Emiliania huxleyi 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 thioredoxin + NADP+
-
 Emiliania huxleyi thioredoxin disulfide + NADPH + H+
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH + H+
-
 Emiliania huxleyi ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 ? x * 52320, isoform SEP1, calculated from amino acid sequence Emiliania huxleyi
1.8.1.9 ? x * 59000, isoform SEP1, SDS-PAGE Emiliania huxleyi

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 SEP1 isoform Emiliania huxleyi
1.8.1.9 thioredoxin reductase
-
 Emiliania huxleyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 NADPH
-
 Emiliania huxleyi

Expression

EC Number Organism Comment Expression
1.8.1.9 Emiliania huxleyi thioredoxin reductase activity gradually increases 4fold within ca. 70 h when cells are transferred to the medium containing 10 nM selenite, selenium is essential for the induction of thioredoxin reductase activity at the translational level but not at the transcriptional level up