EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.39 | the wild type and the DELTAG810 mutant of nNOS are cloned in Escherichia coli strain DH5alpha | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.39 | additional information | the DELTAG810 mutant enzyme shows strongly reduced activity towards nitric oxide synthesis and NADPH oxidation compared to the wild type enzyme | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.39 | Rattus norvegicus | P29476 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.39 | 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Rattus norvegicus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.39 | neuronal nitric-oxide synthase | - |
Rattus norvegicus |
1.14.13.39 | nNOS | - |
Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.39 | 0.033 | - |
nitric oxide | DELTAG810 mutant enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus | |
1.14.13.39 | 0.055 | - |
nitric oxide | DELTAG810 mutant enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus | |
1.14.13.39 | 0.28 | - |
NADPH | DELTAG810 mutant enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus | |
1.14.13.39 | 0.32 | - |
NADPH | DELTAG810 mutant enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus | |
1.14.13.39 | 0.92 | - |
nitric oxide | wild type enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus | |
1.14.13.39 | 1.23 | - |
nitric oxide | wild type enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus | |
1.14.13.39 | 2.33 | - |
NADPH | wild type enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus | |
1.14.13.39 | 2.57 | - |
NADPH | wild type enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.39 | 5,6,7,8-tetrahydro-L-biopterin | - |
Rattus norvegicus | |
1.14.13.39 | Calmodulin | - |
Rattus norvegicus | |
1.14.13.39 | NADPH | - |
Rattus norvegicus |