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Literature summary extracted from

  • Huang, C.H.; Winkler, A.; Chen, C.L.; Lai, W.L.; Tsai, Y.C.; Macheroux, P.; Liaw, S.H.
    Functional roles of the 6-S-cysteinyl, 8alpha-N1-histidyl FAD in glucooligosaccharide oxidase from Acremonium strictum (2008), J. Biol. Chem., 283, 30990-30996.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.B3
-
 Sarocladium strictum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.99.B3
-
 Sarocladium strictum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.99.B3 C130A FAD binding site, FAD covalently attached Sarocladium strictum
1.1.99.B3 H70A FAD binding site, FAD covalently attached Sarocladium strictum
1.1.99.B3 H70A/C130A no activity, lack of essential FAD cofactor Sarocladium strictum
1.1.99.B3 Y310A larger amount of carbohydrates Sarocladium strictum

General Stability

EC Number General Stability Organism
1.1.99.B3 and wild-type proteins are 1.9 M, 2.4 M, 2.6 M, and 3.2 M, respectively. Sarocladium strictum
1.1.99.B3 and wild-type proteins are 48°C, 52°C, 46°C, and 56°C, respectively. Sarocladium strictum
1.1.99.B3 The melting temperatures for the H70A, C130A, H70A/C130A, Sarocladium strictum
1.1.99.B3 The [guanidine HCl]1/2 values for the H70A, C130A, H70A/C130A, Sarocladium strictum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.B3 0.061
-
 cellobiose wild type protein Sarocladium strictum
1.1.99.B3 0.22
-
 cellobiose H70A mutant protein Sarocladium strictum
1.1.99.B3 0.4
-
 cellobiose C130A mutant protein Sarocladium strictum

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.B3 Sarocladium strictum Q6PW77
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.B3 glycoprotein
-
 Sarocladium strictum

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.B3
-
 Sarocladium strictum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.B3 cello-oligosaccharide + acceptor
-
 Sarocladium strictum ?
-
 ?
1.1.99.B3 cellobiose + acceptor
-
 Sarocladium strictum ?
-
 ?
1.1.99.B3 D-glucose + acceptor
-
 Sarocladium strictum ?
-
 ?
1.1.99.B3 lactose + acceptor
-
 Sarocladium strictum ?
-
 ?
1.1.99.B3 malto-oligosaccharide + acceptor
-
 Sarocladium strictum ?
-
 ?
1.1.99.B3 maltose + acceptor
-
 Sarocladium strictum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.B3 glucooligosaccharide oxidase
-
 Sarocladium strictum
1.1.99.B3 GOOX
-
 Sarocladium strictum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.B3 0.22
-
 cellobiose H70A mutant protein Sarocladium strictum
1.1.99.B3 0.58
-
 cellobiose C130A mutant protein Sarocladium strictum
1.1.99.B3 11.9
-
 cellobiose wild type protein Sarocladium strictum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.B3 FAD covalently bound Sarocladium strictum