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Literature summary extracted from

  • Forouhar, F.; Abashidze, M.; Xu, H.; Grochowski, L.L.; Seetharaman, J.; Hussain, M.; Kuzin, A.; Chen, Y.; Zhou, W.; Xiao, R.; Acton, T.B.; Montelione, G.T.; Galinier, A.; White, R.H.; Tong, L.
    Molecular insights into the biosynthesis of the F420 coenzyme (2008), J. Biol. Chem., 283, 11832-11840.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.28 expression in Escherichia coli Methanosarcina mazei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.8.28 crystal structures of the CofD enzyme, in its free form at 3.1 A resolution, in a ternary complex with 7,8-didemethyl-8-hydroxy-5-deazaflavin and phosphate at 2.5 A resolution, and in a ternary complex with 7,8-didemethyl-8-hydroxy-5-deazaflavin and GDP at 3.0 A resolution Methanosarcina mazei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.28 lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin Methanosarcina mazei the reaction is the fourth step in the biosynthesis of coenzyme F420 guanosine 5'-phosphate + coenzyme F420-0 coenzyme F420-0 is coenzyme F420 without glutamic acid ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.28 Methanosarcina mazei Q8PVT6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.28
-
 Methanosarcina mazei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.28 additional information
-
 CofD activity is measured in a coupled assay with lactylphosphate guanylyltransferase (CofC, MJ1117), which produces lactyl-2-diphospho-5'-guanosine from 2-phospho-L-lactate and GTP. The reaction mixture contains 50 mM TES (pH 7.5), 0.1 mM GTP, 10 mM 2-phospho-L-lactate, 0.4 mM 7,8-didemethyl-8-hydroxy-5-deazariboflavin, 2 mM MnCl2, 0.01 mg of CofC, and 0.0004–0.0006 mg of CofD. The enzyme system is inhibited by high concentrations of GMP. Therefore, 2 mM phosphoenolpyruvate and 1 unit of pyruvate kinase are also included in the reaction mixture to regenerate GTP from GMP Methanosarcina mazei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.28 lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin the reaction is the fourth step in the biosynthesis of coenzyme F420 Methanosarcina mazei guanosine 5'-phosphate + coenzyme F420-0 coenzyme F420-0 is coenzyme F420 without glutamic acid ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.28 2-phospho-L-lactate transferase
-
 Methanosarcina mazei
2.7.8.28 CofD
-
 Methanosarcina mazei