EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.98 | residues 483 to 903 of isoform 1 of chHDAC7 are cloned into a modified pET28a vector for expression in Escherichia coli BL21DE3 Codon Plus RIL cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.1.98 | the crystal structures of the catalytic domain of HDAC7 and its complexes with suberoylanilide hydroxamic acid and trichostatin A are solved | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.98 | H843A | mutant, constructed to assess the role of the active site His-843 | Homo sapiens |
3.5.1.98 | H843F | mutant, constructed to assess the role of the active site His-843 | Homo sapiens |
3.5.1.98 | H843Y | mutant, constructed to assess the role of the active site His-843 | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.98 | (2E)-N-hydroxy-3-[3-(phenylsulfamoyl)phenyl]prop-2-enamide | - |
Homo sapiens | |
3.5.1.98 | LAQ824 | - |
Homo sapiens | |
3.5.1.98 | LBH589 | - |
Homo sapiens | |
3.5.1.98 | oxamflatin | - |
Homo sapiens | |
3.5.1.98 | suberoylanilide hydroxamic acid | SAHA | Homo sapiens | |
3.5.1.98 | trichostatin A | TSA | Homo sapiens | |
3.5.1.98 | tubacin | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.98 | 0.013 | - |
Fluor de Lys HDAC substrate | mutant H843Y | Homo sapiens | |
3.5.1.98 | 0.016 | - |
Fluor de Lys HDAC substrate | mutant H843A | Homo sapiens | |
3.5.1.98 | 0.016 | - |
Fluor de Lys HDAC substrate | mutant H843F | Homo sapiens | |
3.5.1.98 | 0.017 | - |
Fluor de Lys HDAC substrate | wild type enzyme | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.98 | Zn2+ | - |
Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.98 | additional information | Homo sapiens | HDACs catalyze the deacetylation of lysine residues in the N-terminal tails of core histones, in addition non-histone proteins may also serve as substrates | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.98 | Homo sapiens | Q8WUI4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.98 | by affinity chromatography on a 5-ml HiTrap Chelating column charged with Ni2+ and by anion exchange chromatography on a Source 30Q column | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.98 | Fluor de Lys HDAC substrate + H2O | commercially available kit | Homo sapiens | ? | - |
? | |
3.5.1.98 | additional information | HDACs catalyze the deacetylation of lysine residues in the N-terminal tails of core histones, in addition non-histone proteins may also serve as substrates | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.98 | class IIa histone deacetylase | - |
Homo sapiens |
3.5.1.98 | HDAC7 | - |
Homo sapiens |
3.5.1.98 | histone deacetylase 7 | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.98 | 0.0002 | - |
Fluor de Lys HDAC substrate | wild type enzyme | Homo sapiens | |
3.5.1.98 | 0.017 | - |
Fluor de Lys HDAC substrate | mutant H843F | Homo sapiens | |
3.5.1.98 | 0.018 | - |
Fluor de Lys HDAC substrate | mutant H843A | Homo sapiens | |
3.5.1.98 | 1.1 | - |
Fluor de Lys HDAC substrate | mutant H843Y | Homo sapiens |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.5.1.98 | 0.00002 | - |
mutant H843Y | Homo sapiens | (2E)-N-hydroxy-3-[3-(phenylsulfamoyl)phenyl]prop-2-enamide | |
3.5.1.98 | 0.00004 | - |
mutant H843Y | Homo sapiens | trichostatin A | |
3.5.1.98 | 0.00013 | - |
mutant H843Y | Homo sapiens | LBH589 | |
3.5.1.98 | 0.0003 | - |
mutant H843Y | Homo sapiens | suberoylanilide hydroxamic acid | |
3.5.1.98 | 0.0003 | - |
wild type | Homo sapiens | trichostatin A | |
3.5.1.98 | 0.0008 | - |
mutant H843Y | Homo sapiens | tubacin | |
3.5.1.98 | 0.0026 | - |
mutant H843Y | Homo sapiens | oxamflatin | |
3.5.1.98 | 0.0036 | - |
mutant H843Y | Homo sapiens | LAQ824 | |
3.5.1.98 | 0.0043 | - |
wild type | Homo sapiens | (2E)-N-hydroxy-3-[3-(phenylsulfamoyl)phenyl]prop-2-enamide | |
3.5.1.98 | 0.0154 | - |
wild type | Homo sapiens | LBH589 | |
3.5.1.98 | 0.098 | - |
wild type | Homo sapiens | oxamflatin | |
3.5.1.98 | 0.113 | - |
wild type | Homo sapiens | suberoylanilide hydroxamic acid | |
3.5.1.98 | 0.225 | - |
wild type | Homo sapiens | tubacin | |
3.5.1.98 | 0.553 | - |
wild type | Homo sapiens | LAQ824 |