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Literature summary extracted from

  • Newton, G.L.; Ta, P.; Bzymek, K.P.; Fahey, R.C.
    Biochemistry of the initial steps of mycothiol biosynthesis (2006), J. Biol. Chem., 281, 33910-33920.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.250 additional information 1D-myo-inositol 1-phosphate is neither a good substrate nor an inhibitor of MshA. Therefore the commercially available 1DL-myo-inositol 1-phosphate can be used as substrate for MshA assays without complications Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.250 0.12
-
 1D-myo-inositol 3-phosphate 37°C Mycolicibacterium smegmatis
2.4.1.250 0.17
-
 UDP-N-acetyl-D-glucosamine 37°C Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate Mycolicibacterium smegmatis 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, mycothiol biosynthesis 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, mycothiol biosynthesis 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.250 Mycolicibacterium smegmatis A0QQY9 mc2155
-
2.4.1.250 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 A0QQY9 mc2155
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.250 4°C, 2 weeks, activity in cell extract is stable Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 1-phosphate at 10% of the activity with 1D-myo-inositol 3-phosphate Mycolicibacterium smegmatis ?
-
 ?
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 1-phosphate at 10% of the activity with 1D-myo-inositol 3-phosphate Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ?
-
 ?
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, mycothiol biosynthesis Mycolicibacterium smegmatis 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate. Little activity is obtained with myo-inositol, 1D-myo-inositol 1-phosphate, or myo-inositol 2-phosphate as the N-acetylglucosamine acceptor. 1D-myo-inositol 1-phosphate is neither a good substrate nor an inhibitor of MshA. Therfore the commercially available 1DL-myo-inositol 1-phosphate can be used as substrate for MshA assays without complications Mycolicibacterium smegmatis 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, mycothiol biosynthesis Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate. Little activity is obtained with myo-inositol, 1D-myo-inositol 1-phosphate, or myo-inositol 2-phosphate as the N-acetylglucosamine acceptor. 1D-myo-inositol 1-phosphate is neither a good substrate nor an inhibitor of MshA. Therfore the commercially available 1DL-myo-inositol 1-phosphate can be used as substrate for MshA assays without complications Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?
2.4.1.250 UDP-N-acetyl-D-glucosamine + myo-inositol 2-phosphate at 10% of the activity with 1D-myo-inositol 3-phosphate Mycolicibacterium smegmatis ?
-
 ?
2.4.1.250 UDP-N-acetyl-D-glucosamine + myo-inositol 2-phosphate at 10% of the activity with 1D-myo-inositol 3-phosphate Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.250 MshA
-
 Mycolicibacterium smegmatis
2.4.1.250 MSMEG0924
-
 Mycolicibacterium smegmatis