EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.305 | medicine | modification of the lipid A moiety of lipopolysaccharide by the addition of the sugar 4-amino-4-deoxy-L-arabinose is a strategy adopted by pathogenic Gram-negative bacteria to evade cationic antimicrobial peptides produced by the innate immune system. L-Ara4N biosynthesis is therefore a potential anti-infective target | Escherichia coli |
2.1.2.13 | medicine | modification of the lipid A moiety of lipopolysaccharide by the addition of the sugar 4-amino-4-deoxy-L-arabinose is a strategy adopted by pathogenic Gram-negative bacteria to evade cationic antimicrobial peptides produced by the innate immune system. L-Ara4N biosynthesis is therefore a potential anti-infective target | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.305 | overexpression of native and selenomethionine decarboxylase and formyltransferase domains of ArnA | Escherichia coli |
2.1.2.13 | overexpression of native and selenomethionine decarboxylase and formyltransferase domains of ArnA | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.305 | crystallization of native and Se-Met decarboxylase protein. Good quality crystals are obtained with a precipitant solution of 3.2 M NaCl, 0.1 M Bistris, pH 5.2, using a drop containing 0.004 ml of protein and 0.004 ml of precipitant equilibrated against a reservoir of 0.1 ml of precipitant. Space group as P4(1)3(2), with cell dimensions a = b = c = 149.4 A, beta = gamma = 90° | Escherichia coli |
2.1.2.13 | crystallization of native and Se-Met decarboxylase protein. Good quality crystals are obtained with a precipitant solution of 3.2 M NaCl, 0.1 M Bistris, pH 5.2, using a drop containing 0.004 ml of protein and 0.004 ml of precipitant equilibrated against a reservoir of 0.1 ml of precipitant. Space group as P4(1)3(2), with cell dimensions a = b = c = 149.4 A, beta = gamma = 90° | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.305 | E434Q | mutant is inactive, suggesting that chemical rather than steric properties of this residue are crucial in the decarboxylation reaction | Escherichia coli |
2.1.2.13 | E434Q | mutant is inactive, suggesting that chemical rather than steric properties of this residue are crucial in the decarboxylation reaction | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.305 | 0.2 | - |
UDP-glucuronate | pH 7.5, 30°C, S433A decarboxylase mutant | Escherichia coli | |
1.1.1.305 | 0.4 | - |
UDP-glucuronate | pH 7.5, 30°C, E434A decarboxylase mutant | Escherichia coli | |
1.1.1.305 | 0.7 | - |
UDP-glucuronate | pH 7.5, 30°C, ArnA decarboxylase domain | Escherichia coli | |
1.1.1.305 | 1.3 | - |
NAD+ | pH 7.5, 30°C, ArnA decarboxylase domain | Escherichia coli | |
1.1.1.305 | 1.4 | - |
NAD+ | pH 7.5, 30°C, S433A decarboxylase mutant | Escherichia coli | |
1.1.1.305 | 1.6 | - |
NAD+ | pH 7.5, 30°C, E434A decarboxylase mutant, E434Q decarboxylase mutant | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.305 | UDP-glucuronate + NAD+ | Escherichia coli | modification of the lipid A moiety of lipopolysaccharide by the addition of the sugar 4-amino-4-deoxy-L-arabinose is a strategy adopted by pathogenic Gram-negative bacteria to evade cationic antimicrobial peptides produced by the innate immune system. The bifunctional enzyme ArnA is required for 4-amino-4-deoxy-L-arabinose biosynthesis and catalyzes the NAD+-dependent oxidative decarboxylation of UDP-glucuronic acid to generate a UDP-4'-keto-pentose sugar and also catalyzes transfer of a formyl group from N-10-formyltetrahydrofolate to the 4'-amine of UDP-4-amino-4-deoxy-L-arabinose | UDP-beta-L-threo-pentapyranos-4-ulose + CO2 + NADH + H+ | - |
? | |
2.1.2.13 | 10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinopyranose | Escherichia coli | modification of the lipid A moiety of lipopolysaccharide by the addition of the sugar 4-amino-4-deoxy-L-arabinose is a strategy adopted by pathogenic Gram-negative bacteria to evade cationic antimicrobial peptides produced by the innate immune system. The bifunctional enzyme ArnA is required for 4-amino-4-deoxy-L-arabinose biosynthesis and catalyzes the NAD+-dependent oxidative decarboxylation of UDP-glucuronic acid to generate a UDP-4'-keto-pentose sugar and also catalyzes transfer of a formyl group from N-10-formyltetrahydrofolate to the 4'-amine of UDP-4-amino-4-deoxy-L-arabinose | 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinopyranose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.305 | Escherichia coli | P77398 | - |
- |
2.1.2.13 | Escherichia coli | P77398 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.305 | UDP-glucuronate + NAD+ | modification of the lipid A moiety of lipopolysaccharide by the addition of the sugar 4-amino-4-deoxy-L-arabinose is a strategy adopted by pathogenic Gram-negative bacteria to evade cationic antimicrobial peptides produced by the innate immune system. The bifunctional enzyme ArnA is required for 4-amino-4-deoxy-L-arabinose biosynthesis and catalyzes the NAD+-dependent oxidative decarboxylation of UDP-glucuronic acid to generate a UDP-4'-keto-pentose sugar and also catalyzes transfer of a formyl group from N-10-formyltetrahydrofolate to the 4'-amine of UDP-4-amino-4-deoxy-L-arabinose | Escherichia coli | UDP-beta-L-threo-pentapyranos-4-ulose + CO2 + NADH + H+ | - |
? | |
1.1.1.305 | UDP-glucuronate + NAD+ | modification of the lipid A moiety of lipopolysaccharide by the addition of the sugar 4-amino-4-deoxy-L-arabinose is a strategy adopted by pathogenic Gram-negative bacteria to evade cationic antimicrobial peptides produced by the innate immune system. The bifunctional enzyme ArnA is required for 4-amino-4-deoxy-L-arabinose biosynthesis and catalyzes the NAD+-dependent oxidative decarboxylation of UDP-glucuronic acid to generate a UDP-4'-keto-pentose sugar and also catalyzes transfer of a formyl group from N-10-formyltetrahydrofolate to the 4'-amine of UDP-4-amino-4-deoxy-L-arabinose. Residues Ser433 and Glu434 of the decarboxylase domain are required for the oxidative decarboxylation of UDP-glucuronate. Decarboxylase domain catalyzes both hydride abstraction (oxidation) from the C-4' position and the subsequent decarboxylation | Escherichia coli | UDP-beta-L-threo-pentapyranos-4-ulose + CO2 + NADH + H+ | - |
? | |
2.1.2.13 | 10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinopyranose | modification of the lipid A moiety of lipopolysaccharide by the addition of the sugar 4-amino-4-deoxy-L-arabinose is a strategy adopted by pathogenic Gram-negative bacteria to evade cationic antimicrobial peptides produced by the innate immune system. The bifunctional enzyme ArnA is required for 4-amino-4-deoxy-L-arabinose biosynthesis and catalyzes the NAD+-dependent oxidative decarboxylation of UDP-glucuronic acid to generate a UDP-4'-keto-pentose sugar and also catalyzes transfer of a formyl group from N-10-formyltetrahydrofolate to the 4'-amine of UDP-4-amino-4-deoxy-L-arabinose | Escherichia coli | 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinopyranose | - |
? | |
2.1.2.13 | 10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinopyranose | modification of the lipid A moiety of lipopolysaccharide by the addition of the sugar 4-amino-4-deoxy-L-arabinose is a strategy adopted by pathogenic Gram-negative bacteria to evade cationic antimicrobial peptides produced by the innate immune system. The bifunctional enzyme ArnA is required for 4-amino-4-deoxy-L-arabinose biosynthesis and catalyzes the NAD+-dependent oxidative decarboxylation of UDP-glucuronic acid to generate a UDP-4'-keto-pentose sugar and also catalyzes transfer of a formyl group from N-10-formyltetrahydrofolate to the 4'-amine of UDP-4-amino-4-deoxy-L-arabinose. The active site of formyltransfer in ArnA includes the key catalytic residues Asn102, His104, and Asp140 | Escherichia coli | 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinopyranose | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.305 | 30 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.305 | 7.5 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.305 | NAD+ | - |
Escherichia coli | |
2.1.2.13 | NAD+ | - |
Escherichia coli |