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Literature summary extracted from

  • Zylberman, V.; Craig, P.O.; Klinkem, S.; Braden, B.C.; Cauerhff, A.; Goldbaum, F.A.
    High order quaternary arrangement confers increased structural stability to Brucella sp. lumazine synthase (2003), J. Biol. Chem., 279, 8093-8101.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.78 expression in Escherichia coli Brucella sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.78
-
 Brucella sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.78 18000
-
 10 * 18000, it is demonstrated by means of solution light scattering and X-ray structural analyses that the enzyme assembles as a very stable dimer of pentamers. A mechanism for dissociation/unfolding of this macromolecular assembly is postulated Brucella sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate Brucella sp. penultimate step of riboflavin biosynthesis 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.5.1.78 guanidine-HCl produces a cooperative and reversible change in the tertiary structure reflected by a decrease in tryptophan fluorescence emission. In addition, guanidine-HCl incubation produces a complete loss of secondary structure of BLS as monitored by CD spectra Brucella sp.
2.5.1.78 urea 8 M, the absence of structural changes indicates that the quaternary arrangement of the enzyme is very stable Brucella sp.

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.78 Brucella sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.78
-
 Brucella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate penultimate step of riboflavin biosynthesis Brucella sp. 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.78 decamer 10 * 18000, it is demonstrated by means of solution light scattering and X-ray structural analyses that the enzyme assembles as a very stable dimer of pentamers. A mechanism for dissociation/unfolding of this macromolecular assembly is postulated Brucella sp.

Synonyms

EC Number Synonyms Comment Organism
2.5.1.78 BLS
-
 Brucella sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.78 20
-
 the higher molecular order of the decameric enzyme increases its stability at 20°C compared with pentameric lumazine synthases Brucella sp.
2.5.1.78 88
-
 melting temperature. The loss of secondary structure is not recovered after slow cooling of the samples, indicating that an irreversible unfolding takes place under these conditions Brucella sp.