EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.90 | H404A | carboxylases W390A, S398A, and H404A have activities similar to that of wild type | Homo sapiens |
4.1.1.90 | L394R | natural mutant, certain individuals with combined deficiencies of vitamin K-dependent proteins have a mutation, L394R, in their gamma-glutamyl carboxylase causing impaired glutamate binding | Homo sapiens |
4.1.1.90 | additional information | Y395A propeptide affinity is similar to that of wild type, but those of L394R and W399A are 16-22fold less than that of wild type. Results of kinetic studies with a propeptide-containing substrate are consistent with results of propeptide binding and FLEEL kinetics. Although propeptide and vitamin K binding in some mutants are affected, our data provide compelling evidence that glutamate recognition is the primary function of the conserved region around Leu394 | Homo sapiens |
4.1.1.90 | S398A | carboxylases W390A, S398A, and H404A have activities similar to that of wild type | Homo sapiens |
4.1.1.90 | W390A | carboxylases W390A, S398A, and H404A have activities similar to that of wild type | Homo sapiens |
4.1.1.90 | W399A | lower activity than wild type | Homo sapiens |
4.1.1.90 | Y395A | lower activity than wild type | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.90 | Boc-(2S,4S)-4-methylglutamic acid-Glu-Val | competitive inhibitor, FLEEL as substrate | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.90 | 0.54 | - |
FLEEL | wild type, pH 7.4, 20°C | Homo sapiens | |
4.1.1.90 | 6.49 | - |
FLEEL | L394R, pH 7.4, 20°C | Homo sapiens | |
4.1.1.90 | 14.8 | - |
FLEEL | Y395A, pH 7.4, 20°C | Homo sapiens | |
4.1.1.90 | 24.3 | - |
FLEEL | W399A, pH 7.4, 20°C | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.1.1.90 | endoplasmic reticulum | rough | Homo sapiens | 5783 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.90 | Homo sapiens | P38435 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.90 | - |
Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.90 | FLEEL + CO2 + O2 + vitamin K hydroquinone | - |
Homo sapiens | ? + vitamin K epoxide + H2O | - |
? | |
4.1.1.90 | L-glutamate + CO2 + O2 + vitamin K hydroquinone | - |
Homo sapiens | gamma-carboxy L-glutamate + vitamin K epoxide + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.90 | carboxylase | - |
Homo sapiens |
4.1.1.90 | gamma-glutamyl carboxylase | - |
Homo sapiens |
4.1.1.90 | glutamate carboxylase | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.90 | additional information | - |
additional information | kcat values relative to wild type are 51% (L394R), 1% (Y395A), and 2% (W399A), pH 7.4, 20°C | Homo sapiens | |
4.1.1.90 | 0.0019 | - |
FLEEL | Y395A, pH 7.4, 20°C | Homo sapiens | |
4.1.1.90 | 0.0033 | - |
FLEEL | W399A, pH 7.4, 20°C | Homo sapiens | |
4.1.1.90 | 0.11 | - |
FLEEL | L394R, pH 7.4, 20°C | Homo sapiens | |
4.1.1.90 | 0.21 | - |
FLEEL | wild type, pH 7.4, 20°C | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.90 | vitamin K | conversion of glutamic acid to gamma-carboxyglutamic acid is coupled with the oxygenation of KH2 to vitamin K 2,3-epoxide and has been referred to as vitamin K epoxidase activity | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.90 | additional information | - |
additional information | Ki value for Boc-(2S,4S)-4-methylglutamic acid-Glu-Val is above 5 mM for W399A, pH 7.4, 20°C | Homo sapiens | |
4.1.1.90 | 0.013 | - |
Boc-(2S,4S)-4-methylglutamic acid-Glu-Val | wild type, pH 7.4, 20°C | Homo sapiens | |
4.1.1.90 | 1.4 | - |
Boc-(2S,4S)-4-methylglutamic acid-Glu-Val | L394R, pH 7.4, 20°C | Homo sapiens | |
4.1.1.90 | 2.1 | - |
Boc-(2S,4S)-4-methylglutamic acid-Glu-Val | Y395A, pH 7.4, 20°C | Homo sapiens |