EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.16 | expression in Escherichia coli | Marichromatium gracile |
1.11.1.17 | expressed in Escherichia coli BL(DE3) | Marichromatium gracile |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.16 | 0.0132 | - |
NADH | pH 7.1, 25°C | Marichromatium gracile | |
1.8.1.16 | 0.097 | - |
glutathione amide disulfide | pH 7.1, 25°C | Marichromatium gracile | |
1.8.1.16 | 1.98 | - |
NADPH | pH 7.1, 25°C | Marichromatium gracile | |
1.8.1.16 | 6.9 | - |
glutathione disulfide | pH 7.1, 25°C | Marichromatium gracile | |
1.11.1.17 | additional information | - |
additional information | kinetic analysis of the glutathione amide reductase, using glutathione amide disulfide (cf. EC 1.8.1.16) and NADH as substrates | Marichromatium gracile |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.1.16 | 49028 | - |
2 * 49028, calculation from sequence | Marichromatium gracile |
1.8.1.16 | 49030 | - |
2 * 49030, nanospray ionization mass spectrometry | Marichromatium gracile |
1.8.1.16 | 98000 | - |
gel filtration | Marichromatium gracile |
1.11.1.17 | 27430 | - |
calculated from the deduced amino acid sequence | Marichromatium gracile |
1.11.1.17 | 27500 | - |
chimeric enzyme composed of one N-terminal peroxidoxin-like domain followed by a glutaredoxin-like C-terminus, SDS-PAGE | Marichromatium gracile |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.16 | glutathione amide disulfide + NADH + H+ | Marichromatium gracile | - |
2 glutathione amide + NAD+ | - |
? | |
1.8.1.16 | glutathione amide disulfide + NADH + H+ | Marichromatium gracile DSM 1712 | - |
2 glutathione amide + NAD+ | - |
? | |
1.8.1.16 | glutathione amide disulfide + NADPH + H+ | Marichromatium gracile | - |
2 glutathione amide + NADP+ | - |
? | |
1.8.1.16 | glutathione amide disulfide + NADPH + H+ | Marichromatium gracile DSM 1712 | - |
2 glutathione amide + NADP+ | - |
? | |
1.8.1.16 | glutathione disulfide + NADH + H+ | Marichromatium gracile | - |
2 glutathione + NAD+ | - |
? | |
1.8.1.16 | glutathione disulfide + NADH + H+ | Marichromatium gracile DSM 1712 | - |
2 glutathione + NAD+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.16 | Marichromatium gracile | - |
- |
- |
1.11.1.17 | Marichromatium gracile | - |
- |
- |
1.11.1.17 | Marichromatium gracile DSM 1712 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.16 | - |
Marichromatium gracile |
1.11.1.17 | partial purification of the Prx/Grx-containing crude extract, centrifugation, ammonium sulfate precipitation, butyl-Sepharose HR 16/10 column, dialysis, ResourceQ column | Marichromatium gracile |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.16 | glutathione amide disulfide + NADH + H+ | - |
Marichromatium gracile | 2 glutathione amide + NAD+ | - |
? | |
1.8.1.16 | glutathione amide disulfide + NADH + H+ | - |
Marichromatium gracile DSM 1712 | 2 glutathione amide + NAD+ | - |
? | |
1.8.1.16 | glutathione amide disulfide + NADPH + H+ | - |
Marichromatium gracile | 2 glutathione amide + NADP+ | - |
? | |
1.8.1.16 | glutathione amide disulfide + NADPH + H+ | - |
Marichromatium gracile DSM 1712 | 2 glutathione amide + NADP+ | - |
? | |
1.8.1.16 | glutathione disulfide + NADH + H+ | - |
Marichromatium gracile | 2 glutathione + NAD+ | - |
? | |
1.8.1.16 | glutathione disulfide + NADH + H+ | - |
Marichromatium gracile DSM 1712 | 2 glutathione + NAD+ | - |
? | |
1.11.1.17 | glutathione amide + H2O2 | similar acitivity with small alkyl hydroperoxides indicating broad substrate specificity | Marichromatium gracile | glutathione amide disulfide + H2O | - |
? | |
1.11.1.17 | glutathione amide + H2O2 | similar acitivity with small alkyl hydroperoxides indicating broad substrate specificity | Marichromatium gracile DSM 1712 | glutathione amide disulfide + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.16 | dimer | 2 * 49028, calculation from sequence | Marichromatium gracile |
1.8.1.16 | dimer | 2 * 49030, nanospray ionization mass spectrometry | Marichromatium gracile |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.16 | GAR | - |
Marichromatium gracile |
1.8.1.16 | garB | - |
Marichromatium gracile |
1.11.1.17 | garB | - |
Marichromatium gracile |
1.11.1.17 | More | glutathione amide reductase, cf. EC 1.8.1.16 | Marichromatium gracile |
1.11.1.17 | Prx-containing peroxidase | - |
Marichromatium gracile |
1.11.1.17 | Prx/Grx chimeric enzyme | peroxidoxin/glutaredoxin chimeric enzyme | Marichromatium gracile |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.17 | 25 | - |
assay at | Marichromatium gracile |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.16 | 0.14 | - |
NADPH | pH 7.1, 25°C | Marichromatium gracile | |
1.8.1.16 | 146 | - |
glutathione disulfide | pH 7.1, 25°C | Marichromatium gracile | |
1.8.1.16 | 186.8 | - |
NADH | pH 7.1, 25°C | Marichromatium gracile | |
1.8.1.16 | 249.7 | - |
glutathione amide disulfide | pH 7.1, 25°C | Marichromatium gracile | |
1.11.1.17 | additional information | - |
additional information | kinetic analysis of the glutathione amide reductase, using glutathione amide disulfide (cf. EC 1.8.1.16) and NADH as substrates | Marichromatium gracile |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.17 | 7.1 | - |
assay at | Marichromatium gracile |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.16 | FAD | formation of a charge-transfer complex between one of the newly reduced active site thiols and the oxidized FAD | Marichromatium gracile | |
1.8.1.16 | NADH | preferred cofactor | Marichromatium gracile | |
1.11.1.17 | FAD | - |
Marichromatium gracile |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.11.1.17 | physiological function | garB encodes the central enzyme in glutathione amide cycling. The enzyme is the first example of a prokaryotic low molecular mass thiol-dependent peroxidase. The hydroperoxide-dependent Prx/Grx-catalyzed oxidation of glutathione amide is established in vitro, and as to be expected, the reaction is fueled by NADH via glutathione amide redox recycling | Marichromatium gracile |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.16 | 0.07 | - |
NADPH | pH 7.1, 25°C | Marichromatium gracile | |
1.8.1.16 | 21.2 | - |
glutathione disulfide | pH 7.1, 25°C | Marichromatium gracile | |
1.8.1.16 | 2574 | - |
glutathione amide disulfide | pH 7.1, 25°C | Marichromatium gracile | |
1.8.1.16 | 14150 | - |
NADH | pH 7.1, 25°C | Marichromatium gracile | |
1.11.1.17 | additional information | - |
additional information | kinetic analysis of the glutathione amide reductase, using glutathione amide disulfide (cf. EC 1.8.1.16) and NADH as substrates | Marichromatium gracile |