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Literature summary extracted from

  • Jordan, D.B.; Bacot, K.O.; Carlson, T.J.; Kessel, M.; Viitanen, P.V.
    Plant riboflavin biosynthesis. Cloning, chloroplast localization, expression, purification, and partial characterization of spinach lumazine synthase (1999), J. Biol. Chem., 274, 22114-22121.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.78 expression in Escherichia coli Spinacia oleracea
2.5.1.78 expression in Escherichia coli Nicotiana tabacum
2.5.1.78 expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.78 0.02
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione
-
 Spinacia oleracea
2.5.1.78 0.026
-
 L-3,4-dihydroxybutan-2-one 4-phosphate
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.78 chloroplast constitutes less than 0.02% of the total chloroplast protein. The enzyme is exclusivelylocated in the chloroplast stroma Spinacia oleracea 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.78 15500
-
 60 * 15500, subunit of mature enzyme, SDS-PAGE Spinacia oleracea
2.5.1.78 16534
-
 60 * 16534, subunit of mature enzyme, calculated from sequence Spinacia oleracea
2.5.1.78 16536
-
 60 * 16536, subunit of mature enzyme, electrospray ionization mass spectrometry Spinacia oleracea
2.5.1.78 850000
-
 gel filtration Spinacia oleracea
2.5.1.78 973000 991000 sedimentaion equilibrium studies Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate Spinacia oleracea lumazine synthase catalyzes the penultimate step of riboflavin biosynthesis 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate Nicotiana tabacum lumazine synthase catalyzes the penultimate step of riboflavin biosynthesis 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate Arabidopsis thaliana penultimate step of riboflavin biosynthesis 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.78 Arabidopsis thaliana
-
-
-
2.5.1.78 Nicotiana tabacum
-
-
-
2.5.1.78 Spinacia oleracea
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.5.1.78 proteolytic modification plant lumazine synthase is synthesized in the cytosol as a larger molecular weight precursor protein, which is post-translationally imported into chloroplasts where it is proteolytically cleaved to its mature size Spinacia oleracea
2.5.1.78 proteolytic modification plant lumazine synthase is synthesized in the cytosol as a larger molecular weight precursor protein, which is post-translationally imported into chloroplasts where it is proteolytically cleaved to its mature size Nicotiana tabacum
2.5.1.78 proteolytic modification plant lumazine synthase is synthesized in the cytosol as a larger molecular weight precursor protein, which is post-translationally imported into chloroplasts where it is proteolytically cleaved to its mature size Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.78 16.5
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Spinacia oleracea 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Nicotiana tabacum 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Arabidopsis thaliana 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate lumazine synthase catalyzes the penultimate step of riboflavin biosynthesis Spinacia oleracea 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate lumazine synthase catalyzes the penultimate step of riboflavin biosynthesis Nicotiana tabacum 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate penultimate step of riboflavin biosynthesis Arabidopsis thaliana 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.78 60-mer 60 * 15500, subunit of mature enzyme, SDS-PAGE Spinacia oleracea
2.5.1.78 60-mer 60 * 16534, subunit of mature enzyme, calculated from sequence Spinacia oleracea
2.5.1.78 60-mer 60 * 16536, subunit of mature enzyme, electrospray ionization mass spectrometry Spinacia oleracea

Synonyms

EC Number Synonyms Comment Organism
2.5.1.78 lumazine synthase
-
 Spinacia oleracea
2.5.1.78 lumazine synthase
-
 Nicotiana tabacum
2.5.1.78 lumazine synthase
-
 Arabidopsis thaliana